论文部分内容阅读
应用ABC-捕获ELISA法研究了包虫病人血清中特异性IgM型和IgE型循环免疫复合物(IgM-CIC,IgE-CIC)。IgM-CIC的检出率为47.6％,IgE-CIC为64.3％。肺包虫病人中CIC的检出率略高于肝包虫病,但无显著性差异。特异性IgE抗体阴性的包虫病人中,IgE-CIC检出率达89.5％,而抗体阳性者中为57.7％。在5例特异性IgM抗体阴性的肺包虫病人中,有4例IgM-CIC阳性,而4例IgM抗体阳性者中仅1例CIC阳性。表明由于CIC的形成可造成特异性IgM和IgE游离抗体的“耗竭”。用8M尿素处理后,三种特异性CIC均可被解离。用单克隆抗体检测解离抗原的阳性率在IgG-CIC中为51.3％,在IgM-CIC中为75.0％,在IgE-CIC中为75.9％,证明解离效率与相应抗体的亲和力呈反比。作者认为,包虫病人特异性抗体的检出,不能反映抗体应答的真正水平,抗体阳性加上抗体阴性而CIC阳性者,才能完全反映抗体应答的频率:在IgG为95.2％,IgM为85.1％,IgE为95.6％。提示包虫病人IgM-CIC和IgE-CIC的检测可大大提高免疫诊断的价值,有实际意义。
The specific IgM and IgE circulating immune complexes (IgM-CIC, IgE-CIC) in sera of patients with hydatid disease were studied by ABC-capture ELISA. The detection rate of IgM-CIC was 47.6% and that of IgE-CIC was 64.3%. The detection rate of CIC in patients with pulmonary hydatid was slightly higher than that of hepatic hydatid disease, but no significant difference. IgE-CIC was detected in 89.5% of IgET-negative echinococcosis patients and 57.7% of antibody-positive patients. Of the 5 patients with specific IgM-negative lung hydatid disease, 4 were IgM-CIC-positive, while only 1 of the 4 IgM-positive patients was positive for CIC. Indicating that “depletion” of specific IgM and IgE free antibodies can result from the formation of CICs. After treatment with 8M urea, all three specific CICs can be dissociated. The positive rate of detection of dissociated antigens by monoclonal antibodies was 51.3% in IgG-CIC, 75.0% in IgM-CIC and 75.9% in IgE-CIC, demonstrating that the dissociation efficiency is inversely proportional to the affinity of the corresponding antibody. The authors concluded that the detection of echinococcosis-specific antibodies did not reflect the true level of antibody response. Antibody-positive plus antibody-negative and CIC-positive patients were able to fully reflect the frequency of antibody responses: IgG at 95.2% and IgM at 85.1 %, IgE 95.6%. Tip hydatid patients IgM-CIC and IgE-CIC detection can greatly enhance the value of immune diagnosis, of practical significance.