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采用固相Ｆｍｏｃ法在自制自动蛋白质化学工作站上合成了其第３位赖氨酸（Ｋ３）被丙氨酸（Ａ）取代的虎纹捕鸟蛛毒素－Ⅰ的突变体Ｋ３Ａ－ＨＷＴＸ－Ⅰ．合成的突变体用Ｅｄｍａｎ降解和电喷雾质谱进行鉴定．Ｋ３Ａ－ＨＷＴＸ－Ⅰ经谷胱甘肽系统进行氧化复性后通过离子交换和反相ＨＰＬＣ纯化．活性分析结果表明，Ｋ３被Ａ取代后ＨＷＴＸ－Ⅰ的生物活性下降了８０％，提示Ｋ３与ＨＷＴＸ－Ⅰ的生物活性有一定的相关性
The mutant K3A-HWTX-Ⅰ of Huwentoxin-Ⅰ, whose third lysine (K3) was replaced by alanine (A), was synthesized by a solid-phase Fmoc method at a home-made automated protein chemistry workstation. Synthetic mutants were identified by Edman degradation and electrospray mass spectrometry. K3A-HWTX-I was oxidatively renaturated by the glutathione system and purified by ion exchange and reverse-phase HPLC. The results of the activity analysis showed that the biological activity of HWTX-I decreased by 80% after K3 was replaced by A, suggesting that there is a certain correlation between K3 and the biological activity of HWTX-I