目的研究神经生长因子（ＮＧＦ）在大鼠体内的药代动力学。方法制备兔抗ＮＧＦ抗体，用双抗体夹心酶联免疫吸附实验（ＥＬＩＳＡ）法测定大鼠血浆ＮＧＦ浓度。结果大鼠ｉｖ３２μｇ·ｋｇ－１后血药浓度时间曲线符合二室开放模型，Ｔ１／２α为０３３０ｈ，Ｔ１／２β为１５ｈ。大鼠ＮＧＦｉｍ３２μｇ·ｋｇ－１后血药浓度时间曲线符合一室一级吸收，血浆ＮＧＦ浓度达峰时间为３７１ｈ，Ｔ１／２（Ｋｅ）为４３０ｈ，２４ｈ吸收百分率为６９１％。结论ＮＧＦ肌注吸收良好，血浆ＮＧＦ浓度可在较长时间内维持在有效浓度以上。 Objective To study the pharmacokinetics of nerve growth factor (NGF) in rats. Methods Rabbit anti-NGF antibody was prepared and the concentration of NGF in rat plasma was determined by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The plasma concentration-time curve of iv 32μg · kg-1 in rats was consistent with the two-compartment open model. The T1 / 2α was 0330h and the T1 / 2β was 15h. The concentration-time curve of plasma NGFim32μg · kg-1 was in accordance with the first-order absorption. The peak time of plasma NGF was 371h, the average time of T1 / 2 (Ke) was 430h and the percentage of 24h absorption was 691 %. Conclusion NGF intramuscularly absorbed well, plasma NGF concentration can be maintained at effective concentrations for a long time.