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微核试验(Micronucleus Test)可作为骨髓细胞的诱变性试验,并能分析染色体缺损。诱变效果的最敏感指标为具有微核的多色红细胞的出现。诱变剂的敏感性则随剂量不同而变化,也取决于所使用的动物种类。本文研究特异性无致病菌(SPF)动物或普通动物对诱变剂环磷酰胺敏感性的差异。实验采用雄性S品系小鼠(每组7～8只)和雌性Wistar大鼠(每组6～10只),饲养于22±3℃、12小时光照/12小时黑暗环境中,SPF动物隔离饲养并证实无致病菌及体内寄生虫。环磷酰胺溶于154mM氯化钠
Micronucleus Test can be used as a mutagenic test for myeloid cells and to analyze chromosomal defects. The most sensitive indicator of mutagenic effect is the appearance of multicolor erythrocytes with micronuclei. The sensitivity of a mutagen varies with the dose, depending on the animal species used. In this paper, we investigated the differences in the sensitivity of specific non-pathogenic bacteria (SPF) animals or common animals to cyclophosphamide. The experiments were conducted in male S strain mice (7 to 8 mice) and female Wistar rats (6 to 10 mice) in a dark environment at 22 ± 3 ° C for 12 hours light / 12 hours, and SPF animals were kept in isolation And confirmed non-pathogenic bacteria and parasites. Cyclophosphamide is dissolved in 154 mM sodium chloride