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目的探讨同源框基因A10(homebox A10 gene,HOXA10)在子宫内膜异位症(endometriosis,EMs)子宫内膜组织中的表达,分析HOXA10在EMs的发病机制。方法选取2013年1月~2014年6月40例经手术病理证实的EMs患者作为研究对象,采集异位和在位内膜组织,并选取40例正常内膜组织作为对照。分别采用实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术检测和蛋白印迹法定量检测HOXA10 mRNA和蛋白的表达,启动子的甲基化检测采用特异性聚合酶链反应(methylation-specific PCR,MSP)。结果 HOXA10 mRNA在EMs异位内膜组织和在位内膜组织中的表达分别为0.63±0.11和0.61±0.09,都明显低于正常内膜的1.18±0.12,差异均具有统计学意义(P<0.05);但异位和在位内膜组织的HOXA10 mRNA表达比较差异无统计学意义(P>0.05)。HOXA10蛋白在EMs异位内膜组织和在位内膜组织中的表达分别为0.49±0.10和0.48±0.11,均明显低于正常内膜的1.45±0.18,差异均具有统计学意义(P<0.05);但异位和在位内膜组织的HOXA10蛋白表达比较差异无统计学意义(P>0.05)。启动子甲基化率在EMs异位内膜组织和在位内膜组织中的表达分别为37.5%(15/40)和15.0%(6/40),都明显高于正常内膜的0.0%,差异均具有统计学意义(P<0.05);但异位和在位内膜组织的启动子甲基化率比较差异无统计学意义(P>0.05)。不同r-AFS分期中异位内膜组织中HOXA10 mRNA、蛋白表达随分期的增加表达下降,启动子甲基化率随分期的增加而升高,差异比较均具有统计学意义(P<0.05)。结论 HOXA10 mRNA和蛋白在EMs患者中呈低表达,可能与启动子甲基化有关。启动子甲基化与EMs的临床进展密切相关。
Objective To investigate the expression of homeobox gene A10 (HOXA10) in endometrial tissue of endometriosis (EMs) and to analyze the pathogenesis of HOXA10 in EMs. Methods From January 2013 to June 2014, 40 patients with pathologically confirmed EMs from January 2013 to June 2014 were enrolled in this study. Ectopic and eutopic endometrial tissues were collected and 40 normal endometrial tissues were selected as control. The expressions of HOXA10 mRNA and protein were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and Western blot respectively. Methylation of the promoter was detected by specific polymerase chain reaction PCR, MSP). Results The expressions of HOXA10 mRNA in ectopic endometrium and eutopic endometrium of EMs were 0.63 ± 0.11 and 0.61 ± 0.09, respectively, which were significantly lower than those of normal endometrium (1.18 ± 0.12, P < 0.05). However, HOXA10 mRNA expression in ectopic and eutopic endometrium showed no significant difference (P> 0.05). The expression of HOXA10 protein in EMs ectopic endometrium and eutopic endometrium were 0.49 ± 0.10 and 0.48 ± 0.11, respectively, which were significantly lower than those in normal endometrium (1.45 ± 0.18, P <0.05) ). However, there was no significant difference in HOXA10 protein expression between ectopic and eutopic endometrium (P> 0.05). The promoter methylation rates were 37.5% (15/40) and 15.0% (6/40) in EMs ectopic endometrium and eutopic endometrium respectively, both of which were significantly higher than those of normal endometrium (0.0% (P <0.05). However, there was no significant difference in promoter methylation between ectopic and eutopic endometrium (P> 0.05). The expression of HOXA10 mRNA and protein in ectopic endometrium of different r-AFS stages decreased with the increase of staging, the promoter methylation rate increased with the increase of staging, the differences were statistically significant (P <0.05) . Conclusions HOXA10 mRNA and protein are low expressed in EMs patients, which may be related to promoter methylation. Promoter methylation is closely related to the clinical progress of EMs.