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通过分散聚合法制备微米级单分散聚甲基丙烯酸缩水甘油酯(PGMA)微球,并对其进行氨基改性,随后在微球内部原位沉积合成磁性纳米粒子,溶胀渗入量子点,最终制备了氨基化、微米级、单分散、超顺磁、荧光复合多功能聚合物微球.通过扫描电子显微镜(SEM)观察微球表面形貌,并计算平均粒径及变异系数,结果显示微球平均粒径为1.42μm,变异系数3.8%.傅里叶红外光谱仪(FTIR)表征证明PGMA微球成功引入氨基.X射线衍射仪(XRD)和振动样品磁强计(VSM)分析表明原位生成了磁性纳米粒子,微球具有超顺磁性.荧光显微镜观察到多功能微球具有较强荧光强度.紫外灯照射下磁分离实验表明微球兼具磁性和荧光.所合成的多功能微球有希望应用于生物分离、生物成像、生物标记和荧光检测等领域.
Microparticles of monodisperse poly (glycidyl methacrylate) glycidyl methacrylate (PGMA) were prepared by dispersion polymerization and amino-modified. Then, the magnetic nanoparticles were deposited in-situ in the microspheres and swollen into the quantum dots to finally prepare Aminofused, micron-sized, monodisperse, superparamagnetic and fluorescent composite multi-functional polymer microspheres were prepared.The microspheres surface morphology was observed by scanning electron microscopy (SEM) and the average particle size and coefficient of variation were calculated.The results showed that the microspheres The average particle size was 1.42 μm and the coefficient of variation was 3.8%. The FTIR characterization proved the successful introduction of amino groups into PGMA microspheres. XRD and VSM analysis showed that in situ formation Magnetic nanoparticles have superparamagnetic microspheres microspheres observed by fluorescence microscopy has a strong fluorescence intensity.Ultraviolet light irradiation magnetic separation experiments show that the microspheres have both magnetic and fluorescent multi-functional microspheres synthesized It is hoped to be applied in the fields of biological separation, bioimaging, biomarker and fluorescence detection.