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目的观察红背叶根对单侧输尿管梗阻(UUO)致大鼠肾间质纤维化的血液流变学及微循环变化的影响,从络病学说探讨其抗肾间质纤维化的中医病机。方法采用单侧输尿管结扎法复制单侧输尿管梗阻致大鼠肾间质纤维化模型,将其随机均分为模型组和红背叶根组,并设假手术组;观察红背叶根对该模型动物全血黏度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、舌下及耳廓脉络变化的影响。结果红背叶根能显著降低肾间质纤维化大鼠的全血黏度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积(HCT)、透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CIV)、血清肌酐(SCr)、尿素氮(BUN)指数,改善纤维化大鼠舌下、耳廓脉络的循环状态。结论红背叶根对肾间质纤维化大鼠血流变和微循环变化的改善,提示活血通络可能是其抗肾间质纤维化的主要病机之一。
Objective To observe the effect of red-backed root on hemorheology and microcirculation of renal interstitial fibrosis induced by unilateral ureteral obstruction (UUO) in rats, and to explore its anti-renal interstitial fibrosis TCM pathology from the theory of collaterals . Methods Unilateral ureteral ligation was used to replicate renal interstitial fibrosis model induced by unilateral ureteral obstruction in rats. The model was randomly divided into model group and red-backed root group, and sham operation group was set up. Red-backed root was observed. The model animals had whole blood viscosity of 150/s shear rate, 30/s shear rate, 5/s shear rate, 1/s shear rate, hematocrit, sublingual and auricular vein network changes. Results Red-backed root can significantly reduce the viscosity of whole blood in rats with renal interstitial fibrosis: 150/s shear rate, 30/s shear rate, 5/s shear rate, 1/s shear rate, and hematocrit (HCT), hyaluronic acid (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV), serum creatinine (SCr), urea nitrogen (BUN) index, improve fibrosis The circulatory state of sublingual and auricular veins in rats. Conclusion The improvement of blood rheology and microcirculation in rats with renal interstitial fibrosis by red-backed roots suggests that Huoxue Tongluo may be one of the main pathogenesis of anti-renal interstitial fibrosis.