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以盆栽‘赤霞珠’葡萄为试验材料,浇施浓度为100 nmol·L~(-1)的外源褪黑素(melatonin)后,在110 n L·L~(-1)臭氧浓度胁迫下,测定葡萄叶片气体交换参数和叶绿素荧光快速诱导动力学曲线,并结合叶绿素荧光淬灭分析葡萄叶片光合性能的变化。结果表明:浇施褪黑素提高了臭氧胁迫下叶绿素a、b和类胡萝卜素的含量以及叶绿素a/b的比值,提高了叶片净光合速率(P_n)和叶片单位面积有活性反应中心的数量(RC/CSm),以及捕获的激子将电子传递到Q_A以后的其他电子受体的概率(Ψ_o),从而缓解了臭氧胁迫下的光抑制程度。结论:外源褪黑素缓解了臭氧胁迫下葡萄叶片叶绿素的降解,通过调节光系统的能量分配,缓解了臭氧胁迫对光合作用的伤害。
After potting ’Cabernet Sauvignon’ grapes as test material, 100 nmol·L -1 exogenous melatonin was applied to the test plant under 110 n L · L -1 ozone concentration , The gas exchange parameters of grape leaves and the fast kinetics curve of chlorophyll fluorescence were measured, and the photosynthetic characteristics of grape leaves were analyzed by combining with chlorophyll fluorescence quenching. The results showed that the application of melatonin increased the content of chlorophyll a, b and carotenoids and the ratio of chlorophyll a / b under ozone stress, increased the net photosynthetic rate (P_n) and the number of active reaction centers per leaf area (RC / CSm), and the probability (Ψ_o) of trapped excitons to transfer electrons to other electron acceptors after Q_A, thus alleviating the degree of photoperiod suppression under ozone stress. Conclusion: Exogenous melatonin alleviates the degradation of chlorophyll in grape leaves under ozone stress. By adjusting the energy distribution of photosystem, the damage of photosynthesis to ozone stress can be alleviated.