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核酸杂交常规方法是用同位素标记已知DMA,用放射自显影显示核酸杂交反应,但是放射性同位素本身的特性影响了它的广泛应用。近年来一些学者试探应用细胞化学方法,用荧光素染料结合DNA。Langer等人用生物素标记的多核苷酸做为核学杂交反应的探针,用于果蝇染色体的研究。在DNA嘌呤环的5位碳原子上结合生物素分子,用切口转移方法,将合生物素的尿嘧啶核苷三磷酸类似物整合入DNA,再用抗生物素抗体与之结合。然后用直接或间接血清学方法检查生物素分子用以显示核酸杂交反应的结果。本文报告生物素标记DNA方法以及用于核酸杂交反应的结果。
The conventional method of nucleic acid hybridization is to label known DMAs with isotopes and display nucleic acid hybridization reactions by autoradiography. However, the characteristics of radioisotopes themselves have influenced its wide application. In recent years, some scholars have tentatively applied cytochemical methods to bind DNA with fluorescein dyes. Langer et al. used biotin-labeled polynucleotides as probes for nuclear hybridization to study Drosophila chromosomes. The biotin molecule is bound to the carbon atom at the 5-position of the DNA purine ring, and the uridine triphosphate analog of biotin is incorporated into the DNA by the nick transfer method, and then combined with the anti-biotin antibody. Biotin molecules are then examined by direct or indirect serological methods to show the results of nucleic acid hybridization reactions. This article reports biotin-labeled DNA methods and the results of nucleic acid hybridization reactions.