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目的 :评价丹参预防及治疗右旋葡聚糖硫酸钠 (DSS)结肠炎小鼠的有效性。方法 :2 0只正常小鼠随机分为两组 ,饮用 DSS7d,同时预防组用丹参 ,对照组用 0 .85 %氯化钠溶液。另 2 0只 DSS诱导的结肠炎小鼠随机分为两组 ,治疗组用丹参 ,对照组用 0 .85 %氯化钠溶液 7d。用疾病活动指数 (DAI)、组织学评分和马休斯猩红蓝(MSB)纤维素染色检测微血栓以评价疗效。结果 :丹参在预防组部分降低微血栓的形成 ,对照组 10例有 6例微血栓阳性 ,预防组 3例阳性。丹参治疗组与对照组的 DAI、直肠、横结肠组织学评分分别为 0 .4 5、0 .4 8(P>0 .0 5 ) ,1.36、1.76 (P<0 .0 5 ) ,1.35、1.6 0 (P<0 .0 5 )。结论 :丹参可能部分抑制微血栓形成和减轻 DSS结肠炎小鼠结肠炎症 ,提示丹参用于溃疡性结肠炎治疗也可能有效。
Objective : To evaluate the effectiveness of Salvia miltiorrhiza in prevention and treatment of mice with dextran sulfate sodium (DSS) colitis. Methods : Twenty normal mice were randomly divided into two groups, drinking DSS7d, while the preventive group used Salvia miltiorrhiza, while the control group used 0.85% sodium chloride solution. The other 20 DSS-induced colitis mice were randomly divided into two groups. The treatment group used Salvia miltiorrhiza and the control group used 0.85% sodium chloride solution for 7 days. Microthrombi were evaluated using disease activity index (DAI), histological scores, and MSH cellulose staining to evaluate efficacy. Results: Salvia miltiorrhiza in the prevention group partially reduced the formation of microthrombus. In the control group, 6 cases had microthrombosis positive in 10 cases and 3 cases in the prevention group. The histological scores of DAI, rectal and transverse colon of Danshen treatment group and control group were 0.45, 0.48 (P>0.05), 1.36, 1.76 (P<0.05), 1.35, 1.6 respectively. 0 (P<0. 0 5 ). Conclusion : Salvia miltiorrhiza may partly inhibit the formation of microthrombus and reduce colonic inflammation in mice with DSS colitis, suggesting that Salvia miltiorrhiza can also be effective in the treatment of ulcerative colitis.