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目的:探讨外用藏药奇正青鹏软膏局部应用治疗大鼠佐剂性关节炎的作用及特点。方法:50只大鼠随机抽取10只作为正常组,其余40只注射弗氏完全佐剂造模后分为模型组、青鹏膏高剂量组(1.6 g·kg-1)、青鹏膏低剂量组(0.4 g·kg-1)。治疗组根据体重分别按1.6,0.4 g·kg-1剂量将药物涂抹于右后肢踝关节周围,连续用药4周。观察青鹏软膏不同剂量对佐剂性关节炎大鼠右后肢肿胀率,血清一氧化氮(NO)、白介素-1β(IL-1β)含量的影响,HE染色镜下观察大鼠踝关节滑膜病理改变。结果:外用藏药青鹏软膏组与模型组比较,大鼠的足肿胀率明显降低(P<0.05),血清NO水平显著降低(P<0.05),病理研究表明青鹏软膏有效抑制佐剂性关节炎大鼠关节滑膜损伤程度,但对血清IL-1β水平没有明显影响。结论:青鹏软膏局部外用对大鼠佐剂性关节炎具有治疗作用。
Objective: To investigate the topical application of topical Tibetan medicine Qi Zheng Qing Peng ointment in the treatment of adjuvant arthritis in rats. Methods: Ten rats were randomly selected as the normal group. The other 40 animals were divided into model group, high-dose Qingpeng cream group (1.6g · kg-1), Qingpeng cream low Dose group (0.4 g · kg-1). According to the weight of the treatment group, the drug was applied to the right hindlimb ankle joint at a dose of 1.6, 0.4 g · kg-1 respectively for 4 weeks. To observe the effect of Qingpeng Ointment on the right hind limb swelling rate, serum nitric oxide (NO) and interleukin-1β (IL-1β) content in rats with adjuvant arthritis at different doses. HE staining was used to observe the synovial membrane Pathological changes. Results: Compared with the model group, the foot swelling rate was significantly decreased (P <0.05) and the level of serum NO was significantly decreased (P <0.05). The pathological studies showed that Qingpeng ointment can effectively inhibit the adjuvant Arthritis synovial injury in rats, but no significant effect on serum IL-1β levels. Conclusion: Topical qingpeng ointment has therapeutic effect on adjuvant arthritis in rats.