论文部分内容阅读
目的研究整合素β3与配体Tenascin-c(TN-c)在乳腺癌组织中的表达,探讨其是否通过p38丝裂原活化蛋白激酶(p38mitogen-activated protein kinase,p38MAPK)激活基质金属蛋白酶-2(MMP-2)。方法本研究应用免疫组化SP方法检测了80例乳腺浸润性导管癌中整合素β3及TN-c的表达,同时在培养的乳腺癌MDA-MB-231细胞系中分别阻断整合素β3和p38MAPK,应用Western Blot的方法,检测p-p38和MMP-2的表达情况。结果整合素β3和TN-c在乳腺浸润性导管癌组织中高表达,整合素β3与TN-c的表达之间存在相关性,并与乳腺癌的TNM分期及淋巴结转移相关。应用整合素β3的抗体抑制表达后,可以降低乳腺癌细胞系MDA-MB-231中p-p38以及MMP-2的表达量;用p38的特异性抑制剂SB203580进行阻断,可以降低MMP-2的表达量。结论乳腺浸润性导管癌中存在整合素β3和TN-c的高表达且它们的表达存在相关性,抑制整合素β3可以通过p38MAPK信号分子,抑制MMP-2的表达。
Objective To investigate the expression of integrin β3 and ligand Tenascin-c (TN-c) in breast cancer tissues and to investigate whether p38 mitogen-activated protein kinase (p38MAPK) activates the expression of matrix metalloproteinase-2 (MMP-2). Methods The immunohistochemical SP method was used to detect the expression of integrin β3 and TN-c in 80 cases of invasive ductal carcinoma of the breast. At the same time, the expression of integrin β3 and TN-c in MDA-MB-231 cells p38MAPK, Western Blot method was used to detect the expression of p-p38 and MMP-2. Results The integrin β3 and TN-c were highly expressed in invasive ductal carcinoma of breast. The expression of integrin β3 and TN-c were correlated with TNM staging and lymph node metastasis in breast cancer. Inhibition of expression of integrin β3 could decrease the expression of p-p38 and MMP-2 in breast cancer cell line MDA-MB-231. Blocking with p38-specific inhibitor SB203580 could reduce the expression of MMP-2 The amount of expression. Conclusion There is a high expression of integrin β3 and TN-c in invasive ductal carcinoma of the breast and their expression is correlated. Inhibition of integrin β3 can inhibit the expression of MMP-2 through the p38 MAPK signaling molecule.