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目的研究湿疮1号对小鼠急性湿疹的抑制作用,探讨其可能的作用机制。方法采用2,4-二硝基氯苯(DNCB)诱导建立小鼠急性湿疹模型,随机分为5组,每组10只,分别为空白对照组(生理盐水)、阳性对照组(注射用氢化可的松琥珀酸钠)及湿疮1号不同剂量(低、中、高剂量)组,另选10只为正常对照组。观察小鼠耳部肿胀度,组织病理改变及血T辅助细胞1(Th1)和2(Th2)型细胞因子的表达情况。结果组织病理变化显示,空白对照组小鼠耳部表皮细胞间及细胞内水肿明显,血管扩张明显,淋巴细胞、血管周围炎性细胞浸润明显,药物干预后,与空白对照组比较,中药高、中剂量组和阳性对照组皮肤细胞间及细胞内水肿减轻,炎性细胞浸润较少。与阳性对照组比较,中药中、低剂量组的肿胀度明显增加,肿胀抑制率明显降低,差异有统计学意义(P<0.05);与正常对照组相比,造模后各组小鼠血清细胞因子IL-2、IFN-γ、IL-1β、TNF-α、IL-5均有明显升高,IL-4水平下降,差异有统计学意义(P<0.05)。干预后,与空白对照相比较,中药各剂量组及阳性对照组IL-2水平下降,IL-4水平升高,差异有统计学意义(P<0.05);中药高、中剂量组及阳性对照组IFN-γ、TNF-α水平下降,差异有统计学意义(P<0.05)。中药各剂量组间比较显示,中、低剂量组IL-2、IFN-γ、TNF-α水平明显高于高剂量组,差异有统计学意义(P<0.05)。结论湿疮1号对急性湿疹有明显的抑制作用,其作用机制可能与调节Th1/Th2细胞因子相关。
Objective To study the inhibitory effect of Shi-Wu-1 on acute eczema in mice and to explore its possible mechanism. Methods Acute eczema in mice was induced by 2,4-dinitrochlorobenzene (DNCB) and divided into 5 groups at random. They were blank control group (saline), positive control group (hydrogenation for injection Cortisone sodium succinate) and wet sore No. 1 (low, middle and high dose), and the other 10 were normal control group. The degree of ear swelling, histopathological changes and the expressions of Th1 and Th2 cytokines in mice were observed. Results Histopathological changes showed that in the blank control group, the ear epidermal cells and intracellular edema were obvious, the vasodilatation was obvious, the infiltration of lymphocytes and perivascular inflammatory cells were obvious. Compared with the blank control group, Middle-dose group and positive control group skin cells and intracellular edema alleviated, infiltration of inflammatory cells less. Compared with the positive control group, the swelling degree of middle and low dose groups of traditional Chinese medicine significantly increased and the inhibition rate of swelling decreased significantly (P <0.05). Compared with the normal control group, The levels of IL-2, IFN-γ, IL-1β, TNF-α and IL-5 were significantly increased and the levels of IL-4 were decreased. The difference was statistically significant (P <0.05). Compared with the blank control group, IL-2 level and IL-4 level in each dose group and positive control group were significantly decreased (P <0.05) after intervention; Compared with the blank control group, The levels of IFN-γ and TNF-α decreased, the difference was statistically significant (P <0.05). The comparison of each dose group showed that the levels of IL-2, IFN-γ and TNF-α in middle and low dose groups were significantly higher than those in high dose groups (P <0.05). Conclusion The wet sores No. 1 has a significant inhibitory effect on acute eczema and its mechanism may be related to the regulation of Th1 / Th2 cytokines.