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为了研究中草药对黄颡鱼源维氏气单胞菌的体外抑菌活性,试验通过水提法提取80种中草药有效成分,采用琼脂平板扩散法和试管二倍稀释法测定80种中草药水提物对维氏气单胞菌TH0426的体外抑菌活性,并研究抗菌活性较强的几种中草药的体外联合抑菌活性。结果表明:80种中草药水提物中对维氏气单胞菌TH0426抑菌效果较好的有五倍子、地榆、射干、鹿衔草、红花、大黄、黄连、姜厚朴,且五倍子与地榆的抑菌活性较稳定,地榆的抑菌及杀菌效果最好,MIC、MBC分别为3.91 mg/m L、31.25 mg/m L;联合抑菌试验表明,五倍子与射干的两两联合抑菌指数(FICI)≤0.5,五倍子分别与黄连、大青叶的两两联合抑菌指数≥0.5~≤1,地榆分别与鹿衔草、姜厚朴的两两联合抑菌指数≥2。说明五倍子与射干为协同作用,五倍子与黄连、大青叶分别为相加作用,地榆与鹿衔草、姜厚朴分别为颉颃作用。
In order to study the antibacterial activity of Chinese herbs against Aeromonas sobria in vitro, 80 kinds of active ingredients of Chinese herbal medicine were extracted by water extraction, and 80 kinds of Chinese herbal medicine aqueous extracts were determined by agar plate diffusion method and test tube double dilution method In vitro antibacterial activity against Aeromonas sobria TH0426 and in vitro antibacterial activity of several Chinese herbal medicines with strong antibacterial activity. The results showed that: 80 Chinese herbal medicine aqueous extracts of Aeromonas sobria TH0426 better antibacterial effect of gallnut, Burnet, Shegan, deer rank grass, safflower, rhubarb, berberine, ginger Magnolia officinalis, and gallnut and Burnet The inhibitory and bactericidal activities were the best. The MIC and MBC were 3.91 mg / mL and 31.25 mg / mL, respectively. Combined antibacterial activity test showed that antibacterial and antibacterial activities of Galla chinensis Index (FICI) ≤ 0.5, respectively, gallnut and Coptis Chuanxiong, each pair of two antibacterial index ≥ 0.5 ~ ≤ 1, respectively, Burnet cephalopods and ginger Magnolia Buplear joint inhibition index ≥ 2. Description gallnut and Shegan as a synergistic effect, gallnut and Coptis, Folium were additive effect, Burnet and deer grass grass, ginger Magnolia were antagonistic effect.