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目的探讨复方丹参对人离体肝组织低温保存下的保护作用。方法采集2001～2005年20例行肝叶切除或肝段切除手术病人的正常肝组织,采用同体对照,分别放入复方丹参液(实验组)及平衡盐液(对照组)中,保存1,3,6h,分别进行常规病理检查,比较相同保存时间实验组和对照组标本的病理改变程度,同时将实验组和对照组各自不同保存时间段标本的病理改变程度进行组内比较。结果实验组不同时间段比较,其中保存1h组与保存3h组标本的病理改变无显著差异,保存6h组与保存3h组及保存1h组标本比较,病理改变有显著性;对照组不同时间段比较,随时间延长,病理改变有显著性;对照组与实验组同时间段比较,病理改变有显著性,且在相同保存时间段内,实验组的病理改变较对照组轻。结论复方丹参对低温保存下的人离体肝组织有保护作用,能延长人离体肝组织保存时间。
Objective To investigate the protective effect of Salvia miltiorrhiza Bunge on protecting human liver tissue from cryopreservation. METHODS: Normal liver tissues from 20 patients undergoing liver resection or hepatectomy were collected from 2001 to 2005, and they were placed in the compound salvia miltiorrhiza solution (experimental group) and balanced salt solution (control group). At 3 and 6 hours, routine pathological examinations were performed to compare the degree of pathological changes between the experimental group and the control group at the same preservation time. At the same time, the degree of pathological changes of the specimens of the experimental group and the control group at different preservation time periods was compared within the group. Results There was no significant difference in the pathological changes between the 1 h group and the 3 h group. There was no significant difference in the pathological changes between the 1 h group and the 3 h group. Compared with the 3 h group and the 1 h group, the pathological changes were significant; the control group was compared at different time periods. With time, the pathological changes were significant. Compared with the experimental group, the pathological changes were significant in the control group and the experimental group. In the same preservation period, the pathological changes in the experimental group were lighter than those in the control group. Conclusion Compound salvia miltiorrhiza has protective effect on human hepatic tissue under cryopreservation and can prolong the preservation time of isolated human liver tissue.