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细胞涂片的染色方法较多,目前对癌细胞的检查多采用巴氏(Papanicolaou)染色法,其优点是细胞核和细胞浆均鲜明而清楚,但操作步骤较多(23个步骤),且染色时间较长(40-50分钟),不能及时诊断,因此我们在临床上摸索了快速染色法,现介绍如下。材料和方法一、材料来源:门诊病人宫颈活检组织印片、宫颈刮片、外阴癌灶及阴道癌灶涂片122例。每例同时涂片2张,用快速和巴氏二种染色法对比,其中34例作了病理对照,病种如表1 二、方法 (一)试剂的配制苏木精液:苏木精2g,95％酒精10ml,铔明矾40g,黄色氧化汞1g,水200ml;2号液:2.5％亮绿(亮绿2.5g、蒸馏水5ml,无水酒精95ml),比原液浓度提高5倍,
Cell smear staining more, the current examination of cancer cells to use Papanicolaou staining method, the advantage of the nucleus and cytoplasm are clear and clear, but more steps (23 steps), and staining A long time (40-50 minutes), can not be diagnosed in time, so we groped in the clinical fast staining, are presented below. Materials and methods First, the source of material: Outpatient cervical biopsy tissue prints, cervical smears, vulvar foci and vaginal smear 122 cases. Each case at the same time smear 2, with fast and Papanicolaou two staining contrast, of which 34 were pathological control, the disease as shown in Table 1 two, the method (a) reagent preparation hematoxylin: hematoxylin 2g, 95% alcohol 10ml, alum 40g, yellow mercury oxide 1g, water 200ml; 2 liquid: 2.5% light green (bright green 2.5g, distilled water 5ml, anhydrous alcohol 95ml)