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目的:比较采用3种不同方法建立的大鼠动脉粥样硬化模型,以确定最佳造模方法。方法:120只健康雄性大鼠,分为空白对照组、高脂饮食+维生素D_3(VD_3)+L-甲硫氨酸(L-met)组、高脂饲料1+VD_3+L-met组、高脂饲料2+VD_3+L-met组。通过检测大鼠血清生化指标和主动脉弓病理切片鉴定各组的动脉粥样硬化病变形成情况。结果:与空白对照组相比,大鼠血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)、内皮素(ET)、白介素lβ(IL-lβ)、白介素8(IL-8)均有不同程度升高;高密度脂蛋白(HDL)、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)则呈现下降趋势。其中高脂饮食+VD3+L-met组指标的变化最显著。各模型组在4,6,8周分别出现动脉粥样硬化早、中、晚期病变,其中高脂饮食+VD_3+L-met组的AS分期病变更典型,造模成功率最高。结论:高脂饮食联合VD_3和L-met灌胃是比较有效的建立动脉粥样硬化大鼠模型的方法。
OBJECTIVE: To compare the rat models of atherosclerosis established by three different methods to determine the optimal modeling method. Methods: One hundred and twenty healthy male rats were divided into blank control group, high fat diet + VD_3 + L-methionine group, high fat diet 1 + VD_3 + L-met group, High-fat diet 2 + VD_3 + L-met group. The pathological changes of atherosclerosis in each group were identified by examining the serum biochemical indexes and aortic arch pathological sections. Results: Compared with the blank control group, the levels of TC, TG, LDL, ET, IL-1β, IL-8) increased to some extent; HDL, SOD, MDA and NO decreased. One of the high-fat diet + VD3 + L-met group indicators of the most significant change. The model group had early, middle and late stages of atherosclerosis at 4, 6, 8 weeks, respectively. The AS staging in hypercholesterol diet + VD_3 + L-met group was more typical and the success rate was the highest. Conclusion: The combination of high fat diet and VD_3 and L-met gavage is an effective method to establish atherosclerosis rat model.