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目的:研究大鼠急性心肌梗死(acutemyocardialinfarction,AMI)后心肌细胞凋亡的发生情况。方法:实验选用105只雌性SD大鼠,随机抽取78只以结扎左冠状动脉前降支的方法制备AMI模型,术后24h存活的43只作为心肌梗死组;另设假手术组(n=27);各组再按观察时点随机分为48h和4周两亚组,即:心肌梗死48h(n=11)和心肌梗死4周(n=13)组,假手术48h(n=10)和假手术4周(n=10)组。末端脱氧核糖核酸转移酶介导的dUTP切口末端标记技术(TUNEL)和DNA凝胶电泳检测心肌细胞凋亡。结果:AMI大鼠梗死区心肌细胞坏死和瘢痕形成的同时,梗死/瘢痕区、梗死边缘区和非梗死区的心肌细胞凋亡指数均显著升高犤心肌梗死48h组分别为(212.86±155.75)/1000,(198.16±120.0)/1000和(63.23±45.43)/1000,心肌梗死4周组分别为(235.14±130.43)/1000,(80.33±44.29)/1000和(31.61±16.39)/1000,P<0.05～0.01犦。结论:大鼠发生AMI后,梗死区心肌细胞坏死和瘢痕形成的同时,梗死/瘢痕区、梗死边缘区和非梗死区均有心肌细胞发生凋亡。
Objective: To investigate the occurrence of cardiomyocyte apoptosis after acute myocardial infarction (AMI) in rats. Methods: 105 female Sprague-Dawley rats were randomly divided into two groups. 78 AMI models were randomly selected to ligate the left anterior descending coronary artery. Among them, 43 survived at 24 hours after MI as myocardial infarction group, and sham operation group (n = 27) ); Each group was divided into 48h and 4 week subgroups according to the observation time, namely: myocardial infarction 48h (n = 11) and myocardial infarction 4weeks (n = 13) And sham operation for 4 weeks (n = 10). End Deoxyribonucleic Acid Transferase-mediated dUTP nick end labeling (TUNEL) and DNA gel electrophoresis were used to detect cardiomyocyte apoptosis. Results: The apoptosis index of myocardial cells in infarct / scar zone, infarct border zone and non-infarct zone increased significantly in AMI rats in myocardial infarction area at 48h (212.86 ± 155.75) / (1000), (198.16 ± 120.0) / 1000 and (63.23 ± 45.43) / 1000, respectively. The levels of myocardial infarction were (235.14 ± 130.43) / 1000, (80.33 ± 44.29) / 1000 and (31.61 ± 16.39) P <0.05 ~ 0.01 犦. CONCLUSION: Myocardial cell apoptosis occurs in infarct / scar area, infarct border area and non-infarct area in AMI rats.