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目的:利用前期已构建WFDC2基因稳定低表达的人浆液性卵巢癌细胞株SKOV3,构建裸鼠皮下移植瘤模型,观察肿瘤生长、转移特点的变化。方法:选择前期构建的稳定株进行体内实验。分组:未处理肿瘤细胞组(OEC组)、转染慢病毒空载体组(OEC-mock组)和转染WFDC2小干扰RNA组(OEC-WFDC2-si组)。组织块移植法建立二代卵巢癌荷瘤裸鼠模型。绘制各组的肿瘤生长曲线,实验终点时比较各组肿瘤的体积和重量,免疫组化检测细胞核增殖抗原(PCNA)表达。结果:OEC-WFDC2-si组皮下移植瘤的生长速度显著慢于OEC组和OEC-mock组,差异有统计学意义(P<0.05)。OECWFDC2-si组首次出现可见肿瘤的时间晚于OEC组和OEC-mock组,差异有统计学意义(P<0.05)。移植后第23天,OEC-WFDC2-si组的肿瘤重量、体积和细胞核增殖抗原(PCNA)表达均低于对照组,差异有统计学意义(P<0.05)。实验组和对照组均未发现淋巴结转移。结论:WFDC2基因沉默显著抑制人卵巢癌SKOV3细胞株在裸鼠体内的生长,WFDC2基因可能在人卵巢浆液性上皮癌进展中起重要作用。
OBJECTIVE: To construct a subcutaneous xenograft model of human ovarian cancer cell line SKOV3 which has been stably expressed with low expression of WFDC2 gene in early stage and observe the changes of tumor growth and metastasis. Methods: Stable strains were selected for in vivo experiments. Grouping: untreated tumor cell group (OEC group), lentiviral empty vector group (OEC-mock group) and WFDC2 small interfering RNA group (OEC-WFDC2-si group). Tissue explant transplantation was used to establish the second generation ovarian cancer bearing tumor model in nude mice. The tumor growth curve of each group was drawn. At the end of the experiment, the tumor volume and weight of each group were compared, and the expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. Results: The growth rate of subcutaneously transplanted tumor in OEC-WFDC2-si group was significantly slower than that in OEC and OEC-mock group (P <0.05). The first visible tumor in OECWFDC2-si group was later than OEC and OEC-mock group (P <0.05). On the 23rd day after transplantation, the tumor weight, volume and the expression of PCNA in OEC-WFDC2-si group were lower than those in control group (P <0.05). No lymph node metastasis was found in the experimental group and the control group. Conclusion: The silencing of WFDC2 significantly inhibits the growth of human ovarian cancer cell line SKOV3 in nude mice, and WFDC2 gene may play an important role in the progression of human ovarian serous epithelial carcinoma.