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目的:评价基因检测手段在病毒性心肌炎(VM C)病原学诊断中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)、地高辛标记CBV-cDNA探针检测了120例心肌炎患者血标本中的柯萨奇B组病毒(CBV)。结果:RT-PCR阳性检出率:心肌炎组为48.33%,对照组4.41%(P<0.01)。斑点杂交阳性检出率:心肌炎组为51.67%,对照组4.41%(P<0.01)。结论:PCR联合地高辛标记探针诊断病毒性心肌炎,不失为一种敏感、特异、简便、可靠的方法。
Objective: To evaluate the role of genetic testing in the etiological diagnosis of viral myocarditis (VM C). METHODS: Coxsackie B virus (CBV) was detected in 120 blood samples from patients with myocarditis using reverse transcription polymerase chain reaction (RT-PCR) and digoxigenin-labeled CBV-cDNA probe. Results: The positive rate of RT-PCR was 48.33% in myocarditis group and 4.41% in control group (P <0.01). The positive rate of dot blot hybridization was 51.67% in myocarditis group and 4.41% in control group (P <0.01). Conclusion: PCR combined with digoxigenin-labeled probe in the diagnosis of viral myocarditis, after all, is a sensitive, specific, simple and reliable method.