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为了简化AC值的测定方法,我们用皂素制备溶血液以代替反复冰冻,缩短操作时间;另外还用5,5-二硫双-2-硝基苯甲酸(简称DTNB)测定GSH,以代替中草酸二酰脲,在国产72型比色汁420mμ处比色,不需要紫外分光光度计,以便在一般实验室操作。经动物和人体试用,简化法测得的AC值与原法基本上无差别。 50头雄性大白鼠,体重65—75g,按窝别分为缺乏、2μg、5μg、10μg、30μg与正常六组。实验期九周。根据体重、尿和肝中核黄素含量,评定各组动物的核黄素营养状况等级。 对照九周后各组动物的AC值,可以将核黄素营养状况的评价标准定为:<1.20为充裕;1.21—1.50中等;1.51—1.80不足;>1.80严重缺乏,出现症状。
In order to simplify the determination of AC value, we used saponin to prepare hemolysis instead of repeated freezing and shorten the operation time. In addition, GSH was measured with 5, 5-dithiobis-2-nitrobenzoic acid (DTNB for short) The oxalic acid oxalate, in the domestic colorimetric type 420 colorimetric 420mμ, do not need UV spectrophotometer for general laboratory operations. Trial by animal and human, simplified method of AC value measured with the original method basically no difference. Fifty male rats weighing 65-75 g were divided into two groups according to their deficiencies: 2 μg, 5 μg, 10 μg, 30 μg and normal six groups. The experimental period of nine weeks. According to body weight, urine and liver riboflavin content, assess the level of riboflavin nutrition status of each group of animals. After nine weeks of comparison of the AC value of each group of animals, riboflavin nutritional status can be assessed as: <1.20 adequate; 1.21-1.50 moderate; 1.51-1.80 inadequate;> 1.80 severe lack of symptoms.