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用 Ｐ Ｃ Ｒ 技术对２７４ 例无肾病并发症的Ⅱ型糖尿病（ Ｎ Ｉ Ｄ Ｄ Ｍ） 、１０２ 例Ⅱ型糖尿病肾病合并肾功能不全（ Ｒ Ｉ） 、１２４ 例Ⅱ型糖尿病肾病无肾功能不全（ Ｎ Ｒ Ｉ） 、２４２ 例正常对照组进行了血管紧张素Ⅰ转换酶（ Ａ Ｃ Ｅ） 基因插入／ 缺失多态性的检测，同时观察血清血管紧张素转换酶（ Ａ Ｃ Ｅ） 水平与 Ａ Ｃ Ｅ 基因多态性及疾病的关系。结果： Ｒ Ｉ组 Ｄ 等位基因频率０ ．５２ ， Ｄ Ｄ 基因型频率０ ．３０ ，与对照组（０ ．３８ ，０ ．１６） 比较有显著差异（ Ｐ＜ ０ ．０５ ， Ｐ＜ ０ ．００１） ； Ｄ Ｄ 基因型较 Ｉ Ｉ对 Ｒ Ｉ 的比数比为２ ．９１（９５ ％ Ｃ Ｉ为１ ．５２ ～５ ．５８ ， Ｐ＜ ０ ．０１） 。 Ｎ Ｉ Ｄ Ｄ Ｍ 组及 Ｎ Ｒ Ｉ组基因型频率分布与对照组比较无显著差异（ Ｐ＞ ０ ．０５） ；各组中 Ｄ Ｄ 基因型个体血清 Ａ Ｃ Ｅ 水平最高 Ｉ Ｉ基因型最低， Ｒ Ｉ组及 Ｎ Ｒ Ｉ组血清 Ａ Ｃ Ｅ 水平显著高于对照组（ Ｐ＜ ０ ．００１） ，基因多态性与血清 Ａ Ｃ Ｅ 水平呈相关性（ ｒ ＝ ０ ．６５ ， Ｐ＜ ０ ．０１） 。表明血清 Ａ Ｃ Ｅ 水平升高对糖尿病肾病的发生及进展有重要作用， Ａ Ｃ Ｅ 基因缺失多态性可能是中
There were 274 cases of type II diabetes without nephropathy (NIDDM), 102 cases of type II diabetic nephropathy with renal insufficiency (RI) and 124 cases of type II diabetic nephropathy without renal insufficiency ( N R I), and 242 normal control subjects were tested for the insertion / deletion polymorphism of angiotensin I converting enzyme (A C E) gene. At the same time, the levels of serum angiotensin converting enzyme (A C E) E gene polymorphism and disease. Results: The D allele frequency in R Ⅰ group was 0. 52, D D genotype frequency 0. 30, which was significantly different from the control group (0.38, 0.16) (P <0.05, P <0.001). The ratio of D D genotype to I I to R I was 2. 91 (95% CI 1.52-5.88, P <0.01). There was no significant difference in genotype distribution between NIDDM and NRI (P> 0.05). In each group, the highest level of AEC in serum of individuals with D & D genotype was the lowest, Serum A C E levels in R I and N R I groups were significantly higher than those in control group (P <0 .001), and genetic polymorphisms were associated with serum C C levels (r = 0.65, P <0. 01). This suggests that elevated serum ACE may play an important role in the pathogenesis and progression of diabetic nephropathy. The deletion of A C E gene may be