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目的 通过检测老年急性冠状动脉综合征(ACS)患者膜联蛋白V(Annexin V)的表达率,来探讨其与心血管疾病危险因素、纤维蛋白原、D-二聚体及血小板聚集率(PAR)的相关性.方法 选取2014年12月至2016年5月在我院行冠状动脉造影者156例,其中ACS 88例,稳定型心绞痛(SAP)36例和冠状动脉造影正常(对照组)32例.采用流式细胞仪检测AnnexinV表达率和血小板聚集率,同时采用免疫荧光法测定纤维蛋白原、D-二-聚体,并详细记录患者的年龄、吸烟史、高血压、糖尿病病史等.比较不同组间AnnexinV表达率,分析其与心血管疾病危险因素、纤维蛋白原、D-二聚体及PAR的相关性.结果 ACS组AnnexinV表达率与SAP组、对照组比较差异均有统计学意义(P＜0.05或P＜0.01),而SAP组与对照组比较差异无统计学意义(P=0.487);当ROC曲线下面积(AUC)为0.876时,cut-off值为29.36％,诊断ACS的敏感性及特异性分别为80.1％、79.9％.ACS患者AnnexinV表达率与血浆纤维蛋白原(r=0.468,P=0.047)、D-二聚体(r=0.451,P=0.040)、PAR(r=0.531,P=0.0I0)、吸烟(r=0.510,P=0.009)、高血压(r=0.506,P=0.012)、糖尿病(r=0.493,P=0.026)呈正相关.结论 老年ACS患者血浆AnnexinV表达率与糖尿病、高血压、吸烟、纤维蛋白原、D-二聚体、PAR呈正相关,对预测ACS的发生及临床风险有一定的参考价值.“,”Aim To detect the plasma Annexin V expression rate in elderly patients with acute coronary syndrome (ACS),and explore the correlation between Annexin V expression rate and cardiovascuar risk factors,fibrinogen (FIB),D-dimer,platelet aggregation rate (PAR).Methods 156 patients who were successfully examined by coronary angiography were recruited in our hospital from December 2014 to May 2016.These patients were divided into ACS group (n=88),stable angina pectoris (SAP) group (n=36) and control group (n=32).The AnnexinVexpression rate and PAR were detected by flow cytometry instrument,the levels of FIB and D-dimer were detected by immunofluorescence method determination.Detailed information were recorded in age,history of smoking,diabetes,hypertension and so on.Different groups were compared to analyse the correlation between the Annexin V expression rate and cardiovascuar risk factors,FIB,D-dimer,PAR.Results The differences of Annexin V expression rate in ACS group were statistically significant compared with SAP group and control group (P＜0.05 or P＜0.01),but there was no statistically significant difference between SAP group and control group (P=0.487).When the area under the ROC curve (AUC) was 0.876,the cut-off point was 29.36％,the sensitivity and specificity of diagnosis of ACS were 80.1％ and 79.9％.The Annexin V expression rate in ACS group had a positive correlation with plasma FIB (r =0.468,P =0.047),D-dimer (r =0.451,P =0.040),PAR (r=0.531,P=0.010),smoking (r=0.510,P=0.009),hypertension (r=0.506,P=0.012) and diabetes (r=0.493,P =0.026).Conclusion The plasma Annexin V expression rate in elderly patients with ACS were positively correlated with diabetes,hypertension,smoking,FIB,D-dimer and PAR,which may be helpful to predict the occurrence and clinical risk of ACS.