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目的 了解血小板衍生生长因子 ( platelet derivedgrowthfactor,PDGF)对肝星状细胞(hepaticstellatecell,HSC)基质分解素 1 (stromelysin 1 ,MMP3)及金属蛋白酶组织抑制因子 1 (tissueinhibitorofmetalloproteinase 1 ,TIMP1 )基因表达的影响。方法 在培养的肝星状细胞系中加入 2 0 0μg/LPDGF ,于 8、2 4、48、72h共 4个时间点收集细胞 ,提取总RNA ;用逆转录 聚合酶链反应(RT PCR)方法测定基质分解素 1及TIMP1 的基因表达水平。结果 PDGF组肝星状细胞基质分解素 1基因表达水平在 8、2 4、48、72h 4个时间点均明显高于对照组 ,48h达高峰 ,为对照组的 2 .5倍。PDGF组肝星状细胞TIMP1 的基因表达水平在 2 4、48、72h亦明显高于对照组 ,2 4h达高峰 ,为对照组的 2倍。结论 PDGF可增强肝星状细胞基质分解素 1及TIMP1 基因的表达
Objective To investigate the effect of platelet derived growth factor (PDGF) on the gene expression of stromelysin 1 (MMP3) and tissue inhibitor of metalloproteinase 1 (TIMP1) in hepatocellulartellate cells (HSC). Methods 200 microg / L PDGF was added into cultured hepatic stellate cell line, and the total RNA was collected at four time points of 8, 24, 48, and 72 hours. The total RNA was extracted. Reverse transcriptase-polymerase chain reaction The gene expression levels of stromelysin 1 and TIMP1 were determined. Results The expression of stromelysin 1 gene in hepatic stellate cells of PDGF group was significantly higher than that of the control group at 8, 2, 48, and 72 hours, reaching the peak at 48 hours, which was 2.5 times of the control group. The gene expression of TIMP1 in hepatic stellate cells of PDGF group was also significantly higher than that of the control group at 2, 48, 72 hours and reached the peak at 24 hours, which was twice that of the control group. Conclusion PDGF can enhance the expression of stromelysin 1 and TIMP1 in hepatic stellate cells