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为进一步探讨黄瓜耐弱光性的特性,对戴多星和津研2号2个黄瓜品种进行弱光胁迫处理(光照强度75~100μmol·m~(-2)·s~(-1)),以600μmol·m~(-2)·s~(-1)光照强度为对照,研究弱光胁迫下其生理生化指标、光合活性、荧光特性、Rubisco活性、Rubisco大小亚基蛋白含量及基因表达的变化。结果表明,弱光处理后,黄瓜气孔发育受到抑制,气孔开张度、气孔密度和气孔指数均有所降低,且津研2号表现更为明显,所有指标均降低20%以上。黄瓜叶片叶绿素含量、植株净光合速率(Pn),气孔导度(Gs),PSⅡ实际量子效率(ΦPSⅡ),Rubisco活力、Rubisco大小亚基蛋白含量及基因表达均明显降低,其中津研2号所有指标均下降30%以上。与津研2号相比,戴多星的色素含量较高,光能捕获和利用能力较强,分配于光合作用的量子产量较高,Rubisco活性、Rubisco大小亚基蛋白含量及大小亚基基因表达较高,因而Pn相对降低较少(30.9%,津研2号下降54.5%),表现出较强的耐弱光性。本研究结果为黄瓜耐弱光机理的深入研究及耐弱光黄瓜品种的选育栽培提供了参考依据。
In order to further investigate the characteristics of cucumber against weak light, two cucumber varieties, Dai Doxing and Jinyan 2, were exposed to low light stress (light intensity 75-100 μmol · m -2 · s -1) The physiological and biochemical indexes, photosynthetic activity, fluorescence characteristics, Rubisco activity, Rubisco subunit protein content and gene expression under low light stress were studied under 600μmol · m -2 s -1 light intensity. Variety. The results showed that after weak light treatment, the stomatal development of cucumber was inhibited, stomatal opening degree, stomatal density and stomatal index decreased, and Jinyan 2 performed more obviously, all the indexes were reduced by more than 20%. The chlorophyll content, plant photosynthetic rate (Pn), stomatal conductance (Gs), PS Ⅱ actual quantum yield (ΦPSⅡ), Rubisco activity, Rubisco subunit protein content and gene expression were all significantly decreased, among which, Jinyan 2 Indicators are down more than 30%. Compared with Jinyan 2, Dayuoxing had higher pigment content, stronger ability of light energy capture and utilization, higher quantum yield for photosynthesis, Rubisco activity, Rubisco size subunit protein content and size subunit gene The expression of Pn was relatively low (30.9%, Jinyan 2 down 54.5%), showing strong resistance to weak light. The results of this study provide a reference for further research on the mechanism of weak light tolerance of cucumber and the breeding of weak light cucumber varieties.