论文部分内容阅读
多囊卵巢综合征(PCOS)是临床上常见的一种内分泌失调性疾病,也是造成无排卵性不孕的重要原因。对多囊卵巢综合征动物模型的研究有10余年,迄今尚未建立起较为理想的模型动物。该研究的目的在于构建猕猴多囊卵巢综合征动物模型,并分析该模型动物的一些主要生殖特性。将6只成年雌性猕猴(6～10a)平均分成2组:PCOS模型组和对照组。模型组动物的建立方法是:在月经周期的第1、3、5天,分别皮下注射丙酸睾丸酮一次,剂量为3.5mg/kg体重;接着在第7、9、11天,分别肌注人绒毛膜促性腺激素一次,剂量是350IU/kg体重;连续注射2个月经周期。对照组动物注射生理盐水。结果显示:PCOS模型组动物呈现出高血清LH和T,分别为(5.35±0.17)IU/L和(7.58±0.14)ng/mL,而且血清LH/FSH值(5.35/1.30=4.12);模型组动物血清FSH、E2和P的含量与对照组无显著差异。腹部B超扫描结果提示,模型组动物卵巢多囊化。对两组动物进行超排处理后,模型组动物卵巢呈明显的过刺激现象,模型组动物胚胎体外培养的囊胚率为23.53%,显著低于对照组(66.67%)(P<0.05),即用丙酸睾丸酮联合人绒毛膜促性腺激素,能够建立PCOS猕猴模型,该模型动物的一些主要生殖特性与人类PCOS相似。
Polycystic ovary syndrome (PCOS) is a common clinical endocrine disorders, is also an important cause of anovulatory infertility. The study of polycystic ovary syndrome animal model for more than 10 years, so far have not established a more ideal model animal. The aim of this study was to construct an animal model of polycystic ovary syndrome in macaque and to analyze some of the major reproductive characteristics of this model animal. Six adult female macaques (6-10 years) were divided equally into 2 groups: PCOS model group and control group. The model animals were established by subcutaneous injection of testosterone propionate on the first, third and fifth days of the menstrual cycle at a dose of 3.5 mg / kg body weight respectively, followed by intramuscular injection on days 7, 9 and 11 Chorionic gonadotropin once, the dose is 350IU / kg body weight; continuous injection of 2 menstrual cycles. Control animals were injected with saline. The results showed that the serum levels of LH and T were (5.35 ± 0.17) IU / L and (7.58 ± 0.14) ng / mL in PCOS model group, and the serum LH / FSH value was 5.35 / 1.30 = 4.12 The contents of FSH, E2 and P in serum of the animals were not significantly different from those of the control group. Abdominal B-scan results suggest that the animal model group polycystic ovary. Overexpression of two groups of animals showed obvious overstimulation. The blastocyst rate of the model group was 23.53%, which was significantly lower than that of the control group (66.67%) (P <0.05) That testosterone propionate combined with human chorionic gonadotropin, can establish PCOS macaque model, the model animal some of the main reproductive characteristics and human PCOS similar.