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对1989~1992年在湖北省内收集的249份婴幼儿腹泻粪便标本用ELISA、CAT试剂盒和核酸电泳(PAGE)等3种方法检测A组轮状病毒。这3种方法各有优点,可基本满足临床检测的需要。共检出7种电泳型、3种血清型的A组轮状病毒,表明近年来该病毒在我省的流行与80年代初相似,仍以血清1型占优势,但有多种电泳型和血清型同时或交替流行。该类病毒的遗传多样性和易变性增加了预防和控制的难度。
A total of 249 samples of diarrhea from infants and young children collected from 1989 to 1992 in Hubei province were tested for rotavirus A by ELISA, CAT kit and nucleic acid electrophoresis (PAGE). These three methods have their own advantages, can basically meet the needs of clinical testing. A total of seven electrophoresis and three serotypes of group A rotavirus were detected, indicating that the virus in recent years in our province and the early 1980s similar epidemic, still serotype 1 dominant, but there are a variety of electrophoresis and Serum simultaneously or alternately. The genetic diversity and variability of this type of virus increase the difficulty of prevention and control.