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目的:用气相色谱一质谱法分析4种不同产地的白肋烟腺毛分泌物化学成分。方法:采用二氯甲烷提取烟叶腺毛分泌物,毛细管柱进行分折,归一化法测定其百分含量,并用气相色谱一质谱法对化学成分进行鉴定。色谱条件:色谱柱为HP-5MS 5% Phenyl Methyl siloxane(30 m×0.25 mm×0.25μm)弹性石英毛细管柱,柱温:80℃(8 min)→(10℃/min200℃)(10 min)→(3℃/min280℃)(10 min);分流进样,分流比20:1;进样温度230℃,汽化室温度300℃。质谱条件:电离方式EI,电子能量70ev,离子源温度200℃,质量扫描范围30一500 AMU。结果:对比各烟叶的总离子流图,进行了组分分析。不同产地白肋烟腺毛分泌物成分的总离子流图有较大共同点,但亦存在不同之处,各组分的相对百分含量不尽相同,共鉴定出52种化合物。结论:本方法稳定可靠,重现性好,适用于烟叶腺毛分泌物的化学成分分折。
OBJECTIVE: To analyze the chemical composition of the burley glands’ hair secretions in four different areas by gas chromatography-mass spectrometry. Methods: The glandular hair secretions were extracted with methylene chloride, separated by capillary column, and their percentages were determined by normalized method. Chemical components were identified by gas chromatography-mass spectrometry. Chromatographic conditions: HP-5MS 5% Phenyl Methyl siloxane (30 m×0.25 mm×0.25 μm) elastic quartz capillary column, column temperature: 80°C (8 min)→(10°C/min200°C) (10 min) → (3°C/min 280°C) (10 min); split injection, split ratio 20:1; injection temperature 230°C, vaporization chamber temperature 300°C. Mass spectrometry conditions: ionization mode EI, electron energy 70 ev, ion source temperature 200° C., mass scan range 30 to 500 AMU. Results: Component analysis was performed comparing the total ion chromatograms for each tobacco leaf. The total ion current maps of burley gland hair secretions from different origins have more in common, but there are also differences. The relative percentages of the components are not the same. A total of 52 compounds were identified. Conclusion: The method is stable and reliable, and has good reproducibility. It is applicable to the chemical composition of glandular hair secretions in tobacco leaves.