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目的:探讨电离辐射诱导Jurkat T细胞损伤过程中细胞周期进程的变化规律。方法:采用流式细胞术(FCM)检测低剂量(0.075 Gy)和较大剂量(0.5、1.0、2.0、4.0及6.0 Gy)X射线照射后Jurkat T细胞周期进程变化的时间-效应关系和剂量-效应关系。结果:时间-效应关系研究发现,2.0 Gy X射线照射后JurkatT细胞相继发生S期延迟和G2期阻滞,S期细胞百分率于照射后立即增加(P<0.001),而G2+M期细胞百分率照射后8 h开始显著增加(P<0.001)。剂量-效应关系研究发现,75 mGy低剂量X射线照射即可诱导JurkatT细胞发生S期延迟(P<0.001)和G2期阻滞(P<0.05);0.5~6.0 Gy较大剂量X射线照射后,Jurkat T细胞发生S期延迟和G2期阻滞。与假照组相比较,G0/G1期细胞百分率显著降低(P<0.001),在0.5~6.0 Gy内具有剂量依赖性,而S期和G2+M期细胞百分率显著升高(P<0.05或P<0.001)。结论:X射线照射可以改变Jurkat T细胞周期进程,诱导其相继发生S期延迟和G2期阻滞,在0.5~6.0 Gy内具有剂量依赖性。
Objective: To investigate the changes of cell cycle progression during ionizing radiation-induced Jurkat T cell injury. Methods: The time-effect relationship and the dose-duration of Jurkat T cell cycle progression after low dose (0.075 Gy) and high dose (0.5, 1.0, 2.0, 4.0 and 6.0 Gy) X-ray irradiation were detected by flow cytometry (FCM) - the relationship between effects. Results: The time-effect relationship showed that S phase and G2 phase arrest occurred in Jurkat T cells after 2.0 Gy X-ray irradiation, the percentage of S phase cells increased immediately after irradiation (P <0.001), while the percentage of G2 + M phase cells Significant increase began 8 h after irradiation (P <0.001). Dose-effect relationship showed that S-phase delay (P <0.001) and G2 arrest (P <0.05) of Jurkat T cells were induced by 75 mGy low-dose X-ray irradiation. , S phase and J G2 arrest in Jurkat T cells. Compared with the sham group, the percentage of cells in G0 / G1 phase was significantly decreased (P <0.001), and dose-dependent in 0.5 ~ 6.0 Gy, while the percentage of cells in S phase and G2 + M phase was significantly increased (P <0.05 or P <0.001). Conclusion: X-ray irradiation can change the cycle of Jurkat T cells and induce S phase and G2 phase arrest in a dose-dependent manner in 0.5 ~ 6.0 Gy.