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目的模拟真实居住环境,探讨不同装修水平胶合板(释放物主要为甲醛)暴露对小鼠学习记忆功能的影响及其作用机制。方法以1m~2胶合板面积/1m~3房间体积为1倍装修水平。雌性BALB/c小鼠,分别暴露于10倍装修水平[甲醛含量为(3．46±0．26)mg/m~3]和3倍装修水平[甲醛含量为(1．03±0．18)mg/m~3],对小鼠进行吸入染毒,5h/d,连续28 d。另设正常对照组[空气吸入,甲醛含量为(0．05±0．02)mg/m~3]。染毒后进行水迷宫游泳训练及测试。取脑组织,用高效液相色谱-电化学检测器(HPLC-ECD)测定脑组织单胺类肾上腺素(E)、去甲肾上腺素(NE)和多巴胺(DA))神经递质水平。结果染毒组动物不同学习及测试阶段通过迷宫时间延长,学习阶段游入盲端(犯错误)次数增加,与对照组比较,差异有统计学意义(P＜0．05)。不同浓度释放物染毒后小鼠脑组织E含量均下降,与对照组比较,差异有统计学意义(P＜0．01)。结论胶合板释放物甲醛对小鼠学习记忆功能有一定影响,脑组织单胺类神经递质含量降低可能为其学习记忆功能受到抑制的生物学基础。
Objective To simulate the real living environment and to explore the effect of different levels of plywood (release substance mainly formaldehyde) exposure on learning and memory in mice and its mechanism. Method 1m ~ 2 plywood area / 1m ~ 3 room volume 1 times the level of renovation. Female BALB / c mice were exposed to 10 times the level of decoration (formaldehyde content of (3.46 ± 0.26) mg / m ~ 3] and 3 times the level of decoration [formaldehyde content of (1.03 ± 0.18 ) mg / m ~ 3], mice were exposed to inhalation, 5h / d, continuous 28 d. Another normal control group [air inhalation, formaldehyde content (0.05 ± 0.02) mg / m ~ 3]. After water maze swimming training and testing. Brain tissue was taken for determination of neurotransmitter levels of monoamine adrenergic (NE) and dopamine (DA) by HPLC-ECD. Results In the learning and testing stages, the labyrinth time was prolonged during the learning and testing stages of the exposed animals. The number of blind spots (mistakes) in the learning stage increased compared with the control group, and the difference was statistically significant (P <0.05). The contents of E in brain tissue of mice exposed to different concentrations of the release substance decreased, compared with the control group, the difference was statistically significant (P <0.01). Conclusions Formaldehyde, a plywood release substance, has some influence on the learning and memory function of mice. The decrease of monoamine neurotransmitters in brain tissue may be the biological basis for the inhibition of learning and memory function.