论文部分内容阅读
目的:探讨白藜芦醇甙对HIBD新生大鼠ERK通路及p-CREB的作用。方法:选择新生7日龄SD大鼠21只,随机分为假手术对照组(NS组)、阴性对照组(NG组)、缺氧缺血组(HI组)、白藜芦醇甙干预组(PD组)。通过结扎左颈总动脉及通入8%的氧气2 h制备新生大鼠缺氧缺血性脑损伤模型,蛋白质免疫印迹法测定缺氧缺血后24 h大鼠海马p-ERK,p-CREB表达。结果:蛋白质免疫印迹结果显示,HI组大鼠海马区的p-ERK,p-CREB蛋白表达相对灰度值明显要高于NS组(P<0.01),而PD组大鼠海马区的p-ERK,p-CREB蛋白表达相对灰度值同HI组和NS组相比均明显增强(P<0.01)。结论:缺氧缺血性脑损伤后,新生大鼠大脑海马区p-ERK及p-CREB表达增加,表明MAPK/ERK信号通路被激活,应用白藜芦醇甙干预能够进一步增强HIBD新生大鼠大脑海马p-ERK及p-CREB的表达,对于缺氧缺血后新生大鼠大脑具有神经元保护作用。白藜芦醇甙可能通过作用于MAPK/ERK/CREB信号转导通路完成神经保护作用。
Objective: To investigate the effect of polydatin on ERK pathway and p-CREB in neonatal HIBD rats. Methods: Twenty-one newborn 7-day-old SD rats were randomly divided into three groups: sham operation group (NS group), negative control group (NG group), hypoxic-ischemic group (HI group), resveratrol intervention group (PD group). The model of hypoxic-ischemic brain damage was established by ligating the left common carotid artery and introducing 8% oxygen for 2 h. The expressions of p-ERK and p-CREB in hippocampus of hippocampus were detected by Western blotting expression. Results: Western blot results showed that the relative gray value of p-ERK and p-CREB protein in hippocampus of HI group was significantly higher than that in NS group (P <0.01) The relative gray value of ERK and p-CREB protein expression were significantly increased compared with HI group and NS group (P <0.01). Conclusion: The expression of p-ERK and p-CREB in the hippocampus of neonatal rats increased after hypoxic-ischemic brain damage, indicating that the MAPK / ERK signaling pathway is activated. The intervention of polydatin could further enhance the expression of p-ERK and p- The expression of p-ERK and p-CREB in the hippocampus of the hippocampus had neuron protective effects on the brain of neonatal rats after hypoxic-ischemic brain damage. Polydatin may exert neuroprotective effect by acting on the MAPK / ERK / CREB signal transduction pathway.