The changes of lycopene content during ripening and senescence of tomato fruit and the relationship between ethylene glycol-bis (EGTA, Ca2+ chelator), verapamil (Vp, Ca2+ channel blockers), trifluoperazine (TFP), chloropromaize (CPZ) (CaM antagonism) and ethylene-induced increase in lycopene content in tomato fruit were investigated. Lycopene content accumulated obviously during ripening and senescence of tomato fruit after harvest at pink stage. Low temperature inhibited but ethylene enhanced the lycopene content.Meanwhile, ethylene also promoted calmodulin (CaM) content in tomato fruit, which was related to the concentration of ethylene. When EGTA, Vp, TFP and CPZ with ethylene were used to treat tomato fruit, ethylene-induced increase in lycopene content could be reversed, indicating that blocking Ca2+ channel in plasma membrane or chelating extracellular Ca2+ or inhibiting the activity of CaM could decrease the action of ethylene, and suggesting that Ca2+-CaM messenger system may be involved in lycopene increase inducedby ethylene.