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采用飞秒激光对活细胞进行手术,研究了其手术分辨率和微损手术对细胞活性的影响。首先建立飞秒激光活细胞手术系统,可以实时对手术中的细胞进行荧光成像。其次,在绿色荧光蛋白标记组蛋白的Hela细胞核内研究手术分辨率,达到了190nm的极限尺寸,同时发现,细胞核的微损不会导致Hela细胞的死亡,为实现细胞核内微区功能研究奠定了基础。最后,飞秒激光对嗅鞘细胞的突起进行切割,5h内损伤的细胞恢复了活性,因此细胞突起的切割可以实现胞外物质的跨膜运输。
Femtosecond laser surgery on living cells, the surgical resolution and micro-damage surgery on the cell activity. First set up femtosecond laser living cell surgery system, real-time fluorescence imaging of cells in surgery. Secondly, we studied the resolution of surgery in Hela nucleus of GFP-labeled histones and reached the limit of 190 nm. At the same time, it was found that the slight damage of nucleus did not lead to the death of Hela cells, which laid the foundation for the study of intracellular micro- basis. Finally, the femtosecond laser cuts the protrusions of the olfactory ensheathing cells, and the damaged cells resume their activity within 5 h. Therefore, the cutting of the cell protrusions can achieve transmembrane transport of the extracellular material.