目的：通过离体及整体实验观察了人和大鼠肠内菌对人参皂甙Ｒｂ１（ＧＲｂ１）的代谢。方法：采用薄层色谱（ＴＬＣ）和电喷雾质谱（ＥＳＩＭＳ）检测ＧＲｂ１及其代谢产物。结果：离体实验表明，ＧＲｂ１容易被大鼠和人消化道菌群代谢，随着代谢时间的延长，相继出现Ｒｄ，Ｒｇ３／Ｆ２，Ｒｈ２／ＣＫ和Ｐｐｄ４种代谢产物。给大鼠ｉｇＧＲｂ１５００ｍｇ·ｋｇ－１后收集４ｈ和６ｈ粪，提取ＧＲｂ１的代谢产物，证明粪中存在Ｒｄ和Ｒｇ３／Ｆ２两种代谢产物。结论：ＧＲｂ１可被人和大鼠肠内菌代谢，其代谢模式为ＧＲｂ１→Ｒｄ→Ｆ２→ｃｏｍｐｏｕｎｄＫ（ＣＫ）→２０（Ｓ）ｐｒｏｔｏｐａｎａｘａｄｉｏｌ（Ｐｐｄ）。 OBJECTIVE: To observe the metabolism of ginsenoside Rb1 (G-Rb1) by human and rat intestinal bacteria in vitro and in vivo. Methods: GRb1 and its metabolites were detected by thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESIMS). Results: In vitro experiments showed that GRb1 was easily metabolized by the digestive tract of rats and humans. With the prolongation of metabolic time, four metabolites of Rd, Rg3/F2, Rh2/CK and Ppd appeared. After igGRb 1500mg·kg-1 was administered to rats, 4h and 6h feces were collected and the metabolites of GRb1 were extracted to prove that there were two metabolites of Rd and Rg3/F2 in feces. Conclusion: GRb1 can be metabolized by human and rat intestinal bacteria. The metabolic pattern is GRb1→Rd→F2→compoundK(CK)→20(S)protopanaxadiol(Ppd).