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目的:建立浙江省沙门菌PFGE指纹图谱资料库,为查明沙门菌食源性疾病污染源,确定各病例之间的病原学关系,及时处理大范围内呈散发特征的沙门菌事件提供科学依据。方法:收集、提取全省历年来在各类食品和腹泻病人中分离的209株沙门菌的DNA,用限制性内切酶Xbal切割,脉冲场凝胶电泳(PFGE)技术建立PFGE指纹图谱,用BioNu-merics V5.1软件UPGMA方法进行聚类分析。结果:209株沙门菌分成10个基因型别160种PFGE基因指纹图谱。其中67株德尔卑沙门菌可分为6个型别35种PFGE指纹图谱,主要集中在1个PFGE指纹图谱型别中。14株肠炎沙门菌分属3个型别8种PFGE指纹图谱,相似系数在0.800~1.000之间。鼠伤寒沙门菌间的PFGE指纹图谱差异较大。结论:PFGE指纹图谱与血清学型别无相关性,该方法在研究沙门菌分子流行病学、确定各病例之间的病原学关系效果良好。
Objective: To establish the PFGE fingerprinting database of Salmonella in Zhejiang Province, and to provide a scientific basis for identifying the source of salmonella foodborne diseases, determining the etiological relationship between the cases, and treating the Salmonella events that are exuding characteristics in a wide range. Methods: DNA of 209 Salmonella isolates isolated from all kinds of food and diarrhea patients in the province were collected and extracted. The PFGE fingerprinting was established by restriction endonuclease Xbal cleavage and pulsed field gel electrophoresis (PFGE) BioNu-merics V5.1 software UPGMA method for cluster analysis. Results: The 209 strains of Salmonella were divided into 10 genotypes of 160 PFGE gene fingerprints. Among them, 67 strains of Salmonella delphi can be divided into 6 types and 35 kinds of PFGE fingerprints, mainly in one type of PFGE fingerprinting. 14 Salmonella enteritidis belonged to 8 genotypes and 8 PFGE fingerprints with similar coefficients between 0.800 and 1.000. PFGE fingerprinting between Salmonella typhimurium is quite different. Conclusion: There is no correlation between PFGE fingerprinting and serological typing. This method is of great value in studying the molecular epidemiology of Salmonella and determining the etiological relationship among different cases.