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为揭示天然林改为毛竹林过程中土壤微生物变化规律,在浙江省湖州市安吉县和长兴县两地选择不同种植历史的粗放经营毛竹林,分层采集0~20和20~40 cm的混合土壤样品,应用PCR-DGGE技术分析土壤细菌和真菌群落结构及多样性变化.结果表明:在马尾松林改种毛竹林或毛竹林入侵杂灌阔叶林形成毛竹纯林过程中,土壤细菌和真菌的群落结构均发生明显变化,且细菌结构对毛竹种植的响应更敏感;随着毛竹生长时间的延长,表层土壤细菌群落表现出抵抗干扰、最后向改种毛竹之前状态恢复的趋势.毛竹种植时间、样地和土层均对土壤细菌和真菌多样性产生显著影响,其中样地和土层的影响明显大于种植时间.土壤性质和细菌、真菌结构的冗余分析结果表明,不同地点、不同土层驱动土壤微生物结构随时间变化的主要因子没有一致规律,且第1、2轴对样地变化的解释率大多低于65.0%,说明除本研究分析的5个土壤化学指标外,可能还有其他土壤理化性质共同驱动微生物结构的变化.
In order to reveal the change law of soil microorganism during the process of natural forest conversion to Phyllostachys pubescens forest, the extensive management bamboo plantations with different planting history were selected in Anji County and Changxing County, Huzhou City, Zhejiang Province. The mixed layers of 0-20 and 20-40 cm Soil samples were collected and analyzed by PCR-DGGE to analyze the changes of community structure and diversity of soil bacteria and fungi.The results showed that during the process of the Pinus massoniana forest reforestation or the disturbance of broad-leaved forest, The structure of bacterial community changed obviously, and the bacterial structure was more sensitive to the bamboo plantation. With the extension of the growth time of bamboo, the surface soil bacterial community showed the resistance to disturbance, and finally recovered to the state before the bamboo was reformed. , And both plots and soil layers had a significant impact on the diversity of soil bacteria and fungi, and the impact of plots and soil layers was significantly greater than that of planting time. The results of the redundancy analysis of soil properties and bacterial and fungal structures showed that different sites, The main factors that led to the change of soil microbial structure with time were not consistent, and the interpretation rates of the first and the second axis were mostly low 65.0%, indicating that in addition to five indicators in this study soil chemical analysis, there may be other physical and chemical properties of soil microbial co-driven structure.