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斑茅在甘蔗育种的利用是现代甘蔗育种种质创新的热点,育种者期望把斑茅中优异的特性通过杂交渗透到甘蔗中。甘蔗与斑茅的F1是斑茅利用研究的难点也是基础。本研究利用基因组原位杂交技术(GISH)分析甘蔗与斑茅的F1染色体构成和核型,探讨甘蔗与斑茅F1染色体的遗传行为。GISH结果表明,甘蔗与斑茅杂交F1的染色体众数68~69条,其中40条来自甘蔗热带种Badila,28~29条来自海南斑茅,未发现有染色体的交换或易位现象。参试材料大部分染色体都属于中部着丝点(m)的染色体,少数为近中部着丝点(sm),YCE95-41核型属2B型,其余的核型都为1B型。甘蔗与斑茅的染色体按n+n的方式传递给F1,本研究结果为斑茅种质在甘蔗育种中的利用及其杂交后代染色体细胞遗传研究提供参考依据。
The utilization of Drupe in sugarcane breeding is a hot spot in the innovation of modern sugarcane breeding and breeders hope to penetrate the excellent characteristics of Drupe by hybridization into sugarcane. F1 cane and dactylis is the use of spot Mao study the difficulty is the foundation. In this study, F1 chromosome composition and karyotype of sugarcane and dactylis mutanis were analyzed by genomic in situ hybridization (GISH), and the genetic behavior of sugarcane and dwarfing F1 chromosome was explored. The results of GISH showed that there were 68 to 69 chromosomes in F1 hybrid between sugarcane and dactylis, of which 40 were from Badila of sugarcane tropical and 28 to 29 from Hainan dactylidae. No chromosomal exchange or translocation was found. Most of the chromosomes in the test material belong to the chromosomes of central centromere (m), a few are mesocarp (sm), YCE95-41 is 2B, and the rest are 1B. The chromosomes of sugarcane and Dactylis glomerata were transferred to F1 by n + n. The results of this study provide reference for the utilization of germplasm resources in sugarcane breeding and chromosome genetic research in hybrid progenies.