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将从处于一定成熟度的香蕉果肉中纯化出的mRNA反转录成cDNA。cDNA纯化后与EcoRI转接头相连，并插入λgt10载体，经体外包装，侵染大肠杆菌C600hfl菌株，从而构建了香蕉果实的cDNA文库。
The mRNA purified from banana pulp at a certain maturity was reverse transcribed into cDNA. After purification, the cDNA was ligated to the EcoRI adapter and inserted into the λgt10 vector. The cDNA of the banana fruit was constructed by in vitro packaging and infecting the Escherichia coli C600hfl strain.