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目的 :建立草苁蓉提取物的特征图谱。方法:采用高效液相色谱法以Agilent C18(4.6×250mm,5μm)色谱柱,乙腈-水梯度洗脱,流速:0~60min 0.5m L·min~(-1),60~110min 0.8m L·min~(-1),检测波长260nm,柱温30℃。结果 :10批草苁蓉提取物特征图谱相似度较高,利用“中药色谱特征图谱相似度评价软件”生成了草苁蓉提取物的对照特征图谱,共有19个特征峰。结论 :采用HPLC特征图谱技术可以有效地对草苁蓉提取物进行质量评价。
Objective: To establish the characteristic map of Cistanche deserticola extract. Methods: High performance liquid chromatography (HPLC) was used on an Agilent C18 column (4.6 × 250 mm, 5 μm) with acetonitrile-water gradient. The flow rate was 0-60min 0.5m L · min -1 and 60-110min 0.8m L · Min ~ (-1), detection wavelength 260nm, column temperature 30 ℃. Results: The similarity of the characteristic maps of 10 batches of Cistanche deserticola extracts was high, and the control characteristic map of the extracts of Cistanche tuberculosis was generated by using “software of similarity evaluation of traditional Chinese medicine chromatographic profiles”, and there were 19 characteristic peaks. Conclusion: The quality of Cistanche deserticola was evaluated by HPLC profiling.