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目的:探讨在转化生长因子-β1(TGF-β1)刺激下,组蛋白去乙酰化酶抑制剂辛二酰苯胺异羟肟酸(SAHA)对人胚肺成纤维细胞系HELF向肌成纤维细胞(MF)分化时,α-SMA蛋白及前胶原蛋白mRNA表达的影响。方法:体外培养人胚肺成纤维细胞系HELF,并根据不同的实验目的分为空白对照组,TGF-β1处理组以及SAHA干预组。细胞处理结束后,用Western blot检测α-SMA表达,RT-PCR检测I、III型前胶原的mRNA表达水平。结果:空白对照组中几乎无α-SMA蛋白表达,TGF-β1处理后α-SMA水平显著增高,而SAHA能有效降低α-SMA的水平。SAHA孵育24h后,能够明显抑制TGF-β1刺激后的细胞表达I型和III型前胶原蛋白mRNA,并且具有明显的剂量依赖性。结论:SAHA能降低TGF-β1诱导HELF细胞向MF转化时α-SMA表达以及前胶原蛋白表达。
OBJECTIVE: To investigate the effect of suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor, on the proliferation of human embryonic lung fibroblast cell line HELF to myofibroblasts stimulated by transforming growth factor-β1 (TGF-β1) (MF) differentiation, α-SMA protein and procollagen mRNA expression. METHODS: Human embryonic lung fibroblast cell line HELF was cultured in vitro and divided into blank control group, TGF-β1 treatment group and SAHA intervention group according to different experimental purposes. After the cells were treated, the expression of α-SMA was detected by Western blot, and the mRNA expression of type I and III procollagen was detected by RT-PCR. Results: There was almost no expression of α-SMA protein in the blank control group, α-SMA level was significantly increased after TGF-β1 treatment, while SAHA could effectively reduce the level of α-SMA. SAHA incubated for 24 h significantly inhibited TGF-β1-stimulated cells expressing type I and type III procollagen mRNAs in a dose-dependent manner. Conclusion: SAHA can reduce the expression of α-SMA and procollagen in transformed HELF cells to MF induced by TGF-β1.