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A set of ＂three high and one ensuring＂ cultivation mode of double cropping rice, the core of which was high panicle bearing tiller rate, high seed setting rate, high grain plumpness and ensuring high quality, was explored through many years of research. In this study, the effect of ＂three high and one ensuring＂ cultivation mode of double cropping rice was compared and investigated by field experiment and multiple location demonstration. The field experiment indicated that ＂three high and one ensuring＂ cultivation mode promoted the vegetative growth during early stage, inhibited the formation of ineffective tillers, promoted the growth of effective tillers and the formation of panicles, improved the panicle bearing tiller rate, in- creased the total number of spikelets, enhanced the seed setting rate and grain plumpness, increased the grain yield by 12.22%-19.73% at highly significant level, and improved the rice quality. Furthermore, the field demonstration also verified the results of field experiment.