目的:探讨急性非淋巴细胞白血病(ANNL)MIC分型及其与预后关系。方法:43例ANLL患者进行形态学分型,采用流式细胞术检测白血病细胞免疫类型,骨髓短期培养,G显带技术分析染色体核型,分析比较MIC结果及其与预后的关系。结果:ANLL患者髓系系列CD13、CD33和MPO的表达最为常见,阳性率分别为100.00%、95.38%和53.49%;干/祖细胞CD38、HLA-DR和CD34的阳性率分别为83.72%、74.42%、74.42%,各系列单抗在FAB各亚型原始细胞的表达水平不同,其中CD34和CD7分别在急性混合细胞白血病(HAL)和M5高表达;细胞遗传学检查染色体异常率为53.49%,其中复杂异常的患者CR率明显低于其他组。2例FAB分型为M0的患者免疫分型为HAI,3例M5和2例M4为伴淋巴系抗原表达的ANLL。结论:形态学结合免疫分型和细胞遗传学检查可以准确地了解白血病细胞的生物学性质,指导临床治疗和判断预后,尤其对于形态学特征不明显的HAL等患者更有价值。 Objective: To investigate the MIC typing of acute non-lymphocytic leukemia (ANNL) and its relationship with prognosis. Methods: 43 cases of ANLL patients were screened for morphological type. The types of leukemia cells were detected by flow cytometry, short-term bone marrow culture and G-banding technique were used to analyze the karyotype. The relationship between MIC and prognosis was analyzed. Results: The positive rates of CD13, CD33 and MPO in myeloid lineages of ANLL patients were the most common, with the positive rates of 100.00%, 95.38% and 53.49%, respectively. The positive rates of CD38, HLA-DR and CD34 in stem / progenitor cells were 83.72% and 74.42 % And 74.42%, respectively. The expression levels of CD34 and CD7 in primary FAB subtypes were different. The expression rates of CD34 and CD7 were high in acute mixed cell leukemia (HAL) and M5, respectively. The abnormality rate of chromosome in cytogenetics was 53.49% CR in patients with complex abnormalities was significantly lower than other groups. Two patients with FAB typing M0 were immunophenotyped with HAI, three with M5 and two with M4 as ANLL with lymphoid antigen expression. Conclusion: Morphology combined with immunophenotyping and cytogenetic examination can accurately understand the biological characteristics of leukemia cells, guide the clinical treatment and prognosis, especially for patients with morphologic features such as HAL.