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目的:对蒡芩慢咽滴丸中牛蒡苷的含量及溶出度进行了测定,以考察其内在的质量。方法:采用十八烷基键合硅胶色谱柱(4.6mm×250mm,5μm),以甲醇-0.2%磷酸溶液(45∶55)为流动相,流速为1.0mL·min-1,检测波长为280nm。并应用浆法,对蒡芩慢咽滴丸中牛蒡苷进行了溶出度测定。结果:牛蒡苷的线性范围为0.48～2.40μg(r=0.9996),平均回收率(n=6)为99.97%(RSD=0.47%);并以含0.5%十二烷基硫酸钠的蒸馏水为溶剂,转速100r·min-1,经45min,溶出度为标示量的75%以上。结论:该方法简单、迅速、可靠;是控制中药复方内在质量的一种可行方法。
OBJECTIVE: To determine the content and dissolution of arctiin in the burdock extract tablet. Methods: Octadecyl-bonded silica gel column (4.6 mm × 250 mm, 5 μm) was used with a mobile phase of methanol-0.2% phosphoric acid solution (45:55) at a flow rate of 1.0 mL · min-1 and a detection wavelength of 280 nm . And the application of pulp method, burdock Qinlian slow drop pills in the determination of arctiin dissolution. The results showed that the linear range of arctiin was 0.48 ~ 2.40μg (r = 0.9996), the average recovery (n = 6) was 99.97% (RSD = 0.47%); and the content of 0.5% sodium dodecyl sulfate in distilled water was Solvent, speed 100r · min-1, after 45min, the dissolution of the marked amount of more than 75%. Conclusion: The method is simple, rapid and reliable. It is a feasible method to control the intrinsic quality of TCM compound.