目的探讨不同神经毒对N27细胞Caspase-3的影响。方法使用不同的神经毒素1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinium,MPP+)和百草枯(Paraquat,PQ)处理N27细胞,四甲基偶氮唑盐[3-(4,5-dimethylthiazzol-2-y1)-2,5-diphenyl-tetrazdium Bromide,MTT]法检测细胞活性及代谢状态;RT-PCR检测凋亡相关基因Caspase-3的转录。结果经MPP+、百草枯作用后N27细胞活性下降,且其下降程度与MPP+、百草枯浓度的升高呈线性关系;MPP+、百草枯作用后N27细胞Caspase-3 mRNA表达水平升高。结论MPP+、百草枯引起N27细胞生长抑制,并可促进N27细胞中Caspase-3的表达和神经细胞凋亡的发生,神经细胞的凋亡可能是引发帕金森病的因素之一。 Objective To investigate the effect of different neurotoxicity on Caspase-3 in N27 cells. Methods N27 cells were treated with different neurotoxins, 1-methyl-4-phenylpyridinium (MPP +) and paraquat (PQ) MTT assay was used to detect the cell viability and metabolic status. The transcription of Caspase-3 was detected by RT-PCR. Results The activity of N27 decreased after MPP + and paraquat treatment, and its decrease was linear with the increase of MPP + and paraquat concentration. The expression of Caspase-3 mRNA in N27 cells was increased after MPP + and paraquat treatment. Conclusions MPP + and paraquat can inhibit the growth of N27 cells and promote the expression of Caspase-3 and apoptosis of N27 cells. The apoptosis of neurons may be one of the factors that cause Parkinson’s disease.