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实验证明,将B_5培养基的大量、微量元素,有机成份分别降至1/2—1/8,以食糖代替医药用纯蔗糖,以自来水代替蒸馏水配制培养基,对葡萄试管苗的生产速度无不良影响,采用微型扦插的组培技术,个别植株可以当年开花挂果,但不能成熟。试管苗移入大田前需移入土钵,在20—25℃室温条件下过渡15天左右。随着气温的增高,由于室内闷热。必须改为露地过渡移栽,然后移入大田,成活率均可达85%以上。通过试验建立了葡萄组培技术与管外无土生根及嫩枝扦插相结合的快速繁殖工艺规程,其方法简单,育苗速度快,成本低,易推广,任何珍稀优品种只需培养少量原种的试管苗后即可利用管外无土生根和入土试管苗的嫩枝扦插,15天左右就可繁殖一批移栽苗,而且数量大。比单一组培技术生产苗的成本可降低14—27倍。
Experiments show that the B_5 medium, the amount of trace elements, organic ingredients were reduced to 1 / 2-1 / 8, with sugar instead of medicine with pure sucrose, tap water instead of distilled water to prepare medium, the grape seedlings production rate Adverse effects, the use of micro-cutting tissue culture technology, individual plants can flowering fruiting, but not mature. Test tube into the field before the need to move into the soil pot, at room temperature 20-25 ℃ under the conditions of about 15 days transition. As the temperature increases, due to indoor hot. Must be replaced by open field transplanting, and then transferred to the field, the survival rate can reach more than 85%. The method of rapid propagation of grape tissue culture combined with soilless rooting and softwood cutting was established through experiments. The method has the advantages of simple method, fast seedling raising speed, low cost and easy popularization. Only a small amount of rare species Of the tube seedlings can be used after the tube outside the soil without rooting and soil test plantlets tender shoots, about 15 days to breed a group of transplanting seedlings, and a large number. The cost of producing seedlings can be 14-27 times lower than that of single tissue culture techniques.