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目的 :检测K ras基因在国人散发性大肠癌中的突变情况 ,探讨K ras基因突变在中国人大肠癌中的特点以及与临床病理参数的关系。方法 :微解剖取正常粘膜组织 15例、癌组织 35例 ,PCR扩增、DNA测序 ,检测K ras第 12、13和 6 1密码子的突变情况。结果 :癌组织K ras突变率为 14 3% (5 / 35 ) ,均发生在第 12密码子 (GGT→GAT)。第 13和 6 1密码子无突变 ,正常粘膜组织无K ras突变。伴有 12密码子突变的大肠癌患者年龄较大 ,大体以隆起型为主 ,均分布在C和D期 ,有较强的侵袭性。结论 :中国人大肠癌K ras突变率为 14 3% ,低于欧美国家且仅发生在第一外显子的 12密码子的第二碱基。大肠正常粘膜无K ras突变。K ras突变与患者的年龄、肿瘤的大体形态和Dukes分期等临床病理参数有关
Objective : To detect the mutation of Kras gene in sporadic colorectal cancer in Chinese population and to investigate the characteristics of K ras gene mutation in Chinese colorectal cancer and its relationship with clinicopathological parameters. Methods : 15 cases of normal mucosal tissues and 35 cases of cancer tissues were microdissected. PCR amplification and DNA sequencing were used to detect mutations in codons 12, 13, and 61 of Kras. RESULTS: The K ras mutation rate of cancer tissue was 14 3% (5 / 35), which all occurred in the 12th codon (GGT → GAT). The 13th and 61st codons have no mutations, and there is no K ras mutation in normal mucosal tissues. The patients with colorectal cancers with 12 codons are older and generally uplifted. They are all distributed in stages C and D and are highly invasive. Conclusion: The K ras mutation rate of colorectal cancer in Chinese is 14 3%, which is lower than that in Europe and the United States and occurs only in the second base of the 12th codon in the first exon. There was no K ras mutation in the normal mucosa of the colon. K ras mutations are associated with clinical pathological parameters such as patient age, tumor gross morphology, and Dukes staging