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目的:评价Bevacizumab抑制大鼠角膜新生血管的效果。方法:碱烧伤诱导角膜新生血管模型,对20只wistar大鼠40眼随机平均分4组。角膜碱烧伤后分别给予结膜下注射药物溶剂和不同剂量Bevacizumab。比较并计算角膜新生血管生长面积。16d后角膜标本行HE检验和免疫组织化学检测VEGF的表达。结果:各用药组与对照组新生血管面积比较差异有统计学意义;治疗组间新生血管面积比较差异没有统计学意义。组织学发现各药物治疗组炎性细胞浸润、新生血管形成均明显轻于对照组。新生血管对照组VEGF染色明显增强,药物注射组表达明显减弱。结论:结膜下注射一定浓度的Bevacizumab对大鼠角膜碱烧伤形成的新生血管有抑制作用。
Objective: To evaluate the effect of Bevacizumab on corneal neovascularization in rats. Methods: The model of corneal neovascularization induced by alkali burn was divided into 4 groups randomly, 40 eyes of 20 wistar rats. After corneal alkali burn, subconjunctival injection of drug solvent and different doses of Bevacizumab were given respectively. Corneal neovascularization area was compared and calculated. The corneal specimens were examined by HE and immunohistochemistry after 16 days. Results: There were significant differences in the area of neovascularization between each drug group and the control group. There was no significant difference in the area of neovascularization between the treatment groups. Histology found that each drug treatment group inflammatory cell infiltration, neovascularization were significantly lighter than the control group. The neovascularization control group VEGF staining was significantly enhanced, the drug injection group was significantly weakened. CONCLUSIONS: Bevacizumab, administered at a concentration below the subconjunctival level, inhibits neovascularization induced by corneal alkali burns in rats.