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目的研究不同浓度1α,25-二羟维生素D3[1,25-(OH)2·D3]0、10-9、10-8、10-7mol/L对体外培养3～4d龄SD大鼠成骨细胞(OB)骨架F-actin、间隙连接通讯(GJIC)及细胞内钙离子浓度([Ca2+]i)的影响。方法1,25-(OH)2·D3作用20min、24h后测定[Ca2+]i;作用24、48h后观察F-actin及GJIC。结果20min时,不同浓度1,25-(OH)2·D3组[Ca2+]i均显著高于对照组;24h时10-9mol/L组[Ca2+]i则显著低于对照组,其余各组间差异不显著。此时10-8、10-7mol/L组大部分细胞变得扁平,F-actin排列较对照组有序,形成应力纤维;48h时,对照组及10-9mol/L组F-actin表达减少,10-9mol/L组GJIC非常显著地弱于对照组,而10-8、10-7mol/L组大部分细胞F-actin表达完好,GJIC均非常显著地强于其余两组。结论高浓度1,25-(OH)2·D3能够维持OB形态,增强OB间通讯,而低浓度1,25-(OH)2·D3抑制细胞间通讯。
Objective To study the effects of different concentrations of 1α, 25-dihydroxyvitamin D3 [1,25- (OH) 2 · D3] 0,10-9,10-8,10-7mol / L on SD rats aged 3 to 4 days F-actin, GJIC and intracellular calcium concentration ([Ca2 +] i) in osteoblasts (OB). Methods 1,25- (OH) 2 · D3 for 20min, 24h after the determination of [Ca2 +] i; the role of 24,48 hours after F-actin and GJIC observed. Results [Ca2 +] i in 1,25- (OH) 2 · D3 group was significantly higher than that in control group at 20min; [Ca2 +] i in 10-9mol / L group was significantly lower than that in control group at 24h, while the other groups The difference is not significant. At this time, most of the cells in the 10-8,10-7 mol / L group became flattened, and the arrangement of F-actin was orderly and formed stress fibers. At 48h, the expression of F-actin in the control group and 10-9mol / L group was decreased , While GJIC in 10-9mol / L group was significantly weaker than that in control group. The expression of F-actin in most cells of 10-8 and 10-7mol / L group was very good. GJIC was significantly stronger than the other two groups. Conclusion High concentration of 1,25- (OH) 2 · D3 can maintain the morphology of OB and enhance the communication between OBs, while low concentration of 1,25- (OH) 2 · D3 inhibits the intercellular communication.