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目的了解儿童淋巴系统恶性肿瘤免疫分型与ＩｇＨ、ＴＣＲγ基因重排的关系。方法应用ＰＣＲ方法检测８１例已做免疫分型的儿童恶性淋巴瘤和急性淋巴细胞性白血病患者的ＩｇＨ和ＴＣＲγ基因重排。结果Ｂ－ＮＨＬ中，ＩｇＨ基因重排的检出率为８２．４％，ＴＣＲγ基因重排的检测率是７６．５％；Ｔ－ＮＨＬ中，ＩｇＨ基因重排率为２５％，ＴＣＲγ基因重排未检出。在Ｂ－ＡＬＬ中ＩｇＨ基因重排检出率为７４％，ＴＣＲγ基因重排检出率为５０％；在Ｔ－ＡＬＬ中ＩｇＨ基因重排检出率为３７．５％，ＴＣＲγ基因重排检出率为５０％；其中，９１％的病例至少出现一种重排。结论应用ＰＣＲ技术，通过对ＩｇＨ和ＴＣＲγ基因重排的检测，不能判断肿瘤细胞源性，对鉴定淋巴细胞增生的性质有重要意义。
Objective To investigate the relationship between immunophenotyping and IgH and TCRγ gene rearrangements in children with lymphoid malignancies. Methods The IgH and TCRγ gene rearrangements of 81 patients with malignant lymphoma and acute lymphoblastic leukemia were detected by PCR. Results In B-NHL, the detection rate of IgH gene rearrangement was 82.4% and the detection rate of TCRγ gene rearrangement was 76.5%. In T-NHL, IgH gene rearrangement rate was 25% and TCRγ gene weight Row not detected. In B-ALL IgH gene rearrangement detection rate was 74%, TCRγ gene rearrangement detection rate was 50%; T-ALL in IgH gene rearrangement detection rate was 37.5%, TCRγ gene rearrangement The rate of 50%; of which, 91% of the cases at least one kind of rearrangement. Conclusions The detection of IgH and TCRγ gene rearrangements by PCR can not judge the tumor cell origin and is of great significance for identifying the nature of lymphocyte proliferation.