论文部分内容阅读
目的探讨用重组表达的侧链二氧酸脱氢酶复合物E_2亚单位(BCOADC-E_2)检测M_2抗体,以早期诊断原发性胆汁性肝硬化(PBC)。方法重组表达BCOADC-E_2,采用酶联免疫吸附试验(ELISA)和免疫印迹法(IBT)来检测PBC患者的M_2抗体,并以健康体检者、自身免疫病患者、其他非胆汁性肝硬化患者为对照组。结果60例肝病患者血清中检测出抗BCOADC-E_2抗体阳性33例,阴性者为27例,阳性率为55%,而健康体检者和疾病对照组血清中M_2抗体检测均为阴性。结论采用重组表达的人侧链二氧酸脱氢酶复合物E_2亚单位检测M_2抗体,有一定的特异性,对于早期诊断PBC有一定的辅助参考作用。
Objective To investigate the detection of M_2 antibody by using the recombinant E 2 subunit of B 2 O 2 (BCOADC-E 2) to diagnose primary biliary cirrhosis (PBC). Methods Recombinant BCOADC-E 2 was used to detect M 2 antibody in PBC patients by enzyme-linked immunosorbent assay (ELISA) and Western blotting (IBT). The positive rates of M_2 antibody in healthy volunteers, patients with autoimmune diseases and other non-biliary cirrhosis patients were Control group. Results The serum levels of anti-BCOADC-E 2 were detected in 33 out of 60 patients with liver disease, and 27 were negative. The positive rate was 55%. However, M 2 antibody in serum was negative in both healthy subjects and control subjects. Conclusions The detection of M_2 antibody by recombinant human E 2 subunit of human side chain D 2 H 2 O 2 has a certain specificity and can be used as an auxiliary reference for the early diagnosis of PBC.